Long non-coding RNA LINC01296 is a potential prognostic biomarker in patients with colorectal cancer
Colorectal cancer (CRC), one of the most malignant cancers, is currently the fourth leading cause of cancer deaths worldwide. Recent studies indicated that long non-coding RNAs (lncRNAs) could be robust molecular prognostic biomarkers that can refine the conventional tumor-node-metastasis staging system to predict the outcomes of CRC patients. In this study, the lncRNA expression profiles were analyzed in five datasets (GSE24549, GSE24550, GSE35834, GSE50421, and GSE31737) by probe set reannotation and an lncRNA classification pipeline. Twenty-five lncRNAs were differentially expressed between CRC tissue and tumor-adjacent normal tissue samples. In these 25 lncRNAs, patients with higher expression of LINC01296, LINC00152, and FIRRE showed significantly better overall survival than those with lower expression (P < 0.05), suggesting that these lncRNAs might be associated with prognosis. Multivariate analysis indicated that LINC01296 overexpression was an independent predictor for patients’ prognosis in the test datasets (GSE24549, GSE24550) (P = 0.001) and an independent validation series (GSE39582) (P = 0.027). Our results suggest that LINC01296 could be a novel prognosis biomarker for the diagnosis of CRC.
KeywordsColorectal cancer lncRNA Biomarker LINC01296
This work was supported by grants from the National Basic Research Project of China (2010CB529502 and 2007CB511904), National Natural Science Foundation of China (81471485), and the Key Program for the Fundamental Research of the Science and Technology Commission of Shanghai (11JC1411000).
Conflicts of interest
- 3.Sung JJY, Ng SC, Chan FKL, Chiu HM, Kim HS, Matsuda T, et al. An updated Asia Pacific Consensus Recommendations on colorectal cancer screening. Gut. 2014Google Scholar
- 7.Boland CR, Thibodeau SN, Hamilton SR, Sidransky D, Eshleman JR, Burt RW, et al. A National Cancer Institute workshop on microsatellite instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res. 1998;58:5248–57.PubMedGoogle Scholar
- 13.Zhang X, Sun S, Pu JKS, Tsang ACO, Lee D, Man VOY, et al. Long non-coding RNA expression profiles predict clinical phenotypes in glioma. 2012;48:1–8.Google Scholar
- 17.Kim H, Lee D, Yim G, Nam E, Kim S, Kim S, et al. Long non-coding RNA HOTAIR is associated with human cervical cancer progression. Int J Oncol. 2014Google Scholar
- 22.Aziz MA, Periyasamy S, Al YZ, AlAbdulkarim I, Al OM, Alfahed A, et al. Integrated exon level expression analysis of driver genes explain their role in colorectal cancer. PLoS One. 2014;9:e110134.Google Scholar
- 28.Stacklies W, Redestig H, Scholz M, Walther D, Selbig J. PcaMethods a bioconductor package providing PCA methods for incomplete data. 2007;23:1164–1167.Google Scholar
- 30.Ge X, Chen Y, Liao X, Liu D, Li F, Ruan, H, et al. Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer. Med Oncol. 2013;30.Google Scholar
- 36.Pang EJ, Yang R, Fu XB, Liu YF. Overexpression of long non-coding RNA MALAT1 is correlated with clinical progression and unfavorable prognosis in pancreatic cancer. Tumour Biol. 2014Google Scholar
- 39.Qi P, Xu MD, Ni SJ, Shen XH, Wei P, Huang D, et al. Down-regulation of ncRAN, a long non-coding RNA, contributes to colorectal cancer cell migration and invasion and predicts poor overall survival for colorectal cancer patients. Mol Carcinog. 2014.Google Scholar