Tumor Biology

, Volume 36, Issue 9, pp 7277–7283 | Cite as

RETRACTED ARTICLE: MiR-34a suppresses ovarian cancer proliferation and motility by targeting AXL

  • Rui Li
  • Xuejun Shi
  • Fengyu Ling
  • Chunguang Wang
  • Junxia Liu
  • Wei Wang
  • Ming Li
Research Article

Abstract

Increasing evidence has suggested that dysregulation of microRNAs (miRNAs) could contribute to tumor progression. The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in various cancers; however, the effect of miR-34a on the ovarian cancer cells remains unclear. The aim of the paper was to study the expression of miR-34a in ovarian cancer and miR-34a’s relation to the cell proliferation and metastasis in ovarian cancer in vitro. miR-34a expression was determined by quantitative RT-PCR in a panel of 60 human ovarian cancer samples. Functional characterization of miR-34a was accomplished by reconstitution of miR-34a expression in ovarian cancer cells by determining changes in proliferation, migration, and invasion. Our results showed that miR-34a is downregulated in ovarian cancer tissues compared with the corresponding adjacent non-neoplastic tissues, and the expression level of miR-34a was significantly lower in ovarian cancer cell lines in comparison with normal human fallopian tube epithelial cell line. The 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay revealed significant cell proliferation inhibition in miR-34a transfectant compared with the control from HO8910 and SKOV3 cells, which displayed lowest expressions of miR-34a. Furthermore, the transwell assay also showed significant cell migration inhibition in miR-34a transfectant, compared with cell lines transfected with NC. Overexpression of miR-34a led to the inhibition of AXL expression, indicating that AXL is a target gene for miR-34a. Our data suggest that miR-34a may function as a tumor suppressor through repression of oncogenic AXL in ovarian cancer.

Keywords

Ovarian cancer MiR-34a AXL P53 Proliferation Migration 

Notes

Acknowledgments

This study was supported by the grants from the medical research project of Chongqing Municipal Health Bureau (No. 2012-2-159)

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Rui Li
    • 1
  • Xuejun Shi
    • 1
  • Fengyu Ling
    • 1
  • Chunguang Wang
    • 1
  • Junxia Liu
    • 1
  • Wei Wang
    • 1
  • Ming Li
    • 1
  1. 1.Department of OncologyYongchuan Hospital Affiliated to Chongqing Medical UniversityChongqingChina

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