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Tumor Biology

, Volume 36, Issue 9, pp 6759–6764 | Cite as

Role of Lgr5-positive cells in colorectal cancer

  • Honghua Ding
  • Chungang WangEmail author
Research Article

Abstract

The molecular regulation of the growth of colorectal cancer (CRC) cells is not completely understood. Here, we report expression of Lgr5, a stem cell marker for the intestine and hair follicle, in some of the CRC cells in the patients. To determine the role of Lgr5-positive cells in the tumorigenesis of CRCs, we prepared an adeno-associated virus (AAV) that carries diphtheria toxin fragment A (DTA) under the control of Lgr5 promoter (AAV-pLgr5-DTA). Transduction of several CRC cell lines with this virus selectively killed Lgr5-positive cells, resulting in significant inhibition of the CRC cell growth in vitro and in vivo. Thus, our data highlight a potential role of Lgr5-positive cells in the tumorigenesis of CRCs, and suggest that treating these Lgr5-positive cells in CRCs may substantially improve the outcome of CRC therapy.

Keywords

Lgr5 Colorectal cancer Diphtheria toxin fragment A (DTA) Tumorigenesis 

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of Oncology, Shanghai First People’s Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of Radiation Oncology, Shanghai First People’s Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina

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