Tumor suppressive microRNA-200a inhibits renal cell carcinoma development by directly targeting TGFB2
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A large body of evidence indicates that microRNAs play a critical role in tumor initiation and progression by negatively regulating oncogenes or tumor suppressor genes. Here, we report that the expression of miR-200a was notably downregulated in 45 renal cell carcinoma (RCC) samples. Restoration of miR-200a suppressed cell proliferation, migration, and invasion in two RCC cell lines. Furthermore, we used an epithelial-to-mesenchymal transition PCR array to explore the putative target genes of miR-200a. By performing quantitative real-time PCR, ELISA, and luciferase reporter assays, transforming growth factor beta2 (TGFB2) was validated as a direct target gene of miR-200a. Moreover, siRNA-mediated knockdown of TGFB2 partially phenocopied the effect of miR-200a overexpression. These results suggest that miR-200a suppresses RCC development via directly targeting TGFB2, indicating that miR-200a may present a novel target for diagnostic and therapeutic strategies in RCC.
KeywordsmiR-200a Tumor suppressor TGFB2 Renal cell carcinoma
This project was supported by Science and Technology Planning Project of Shenzhen in China (no. JCYJ20140415162543037) and Health and Family Planning Scientific Research Project of Shenzhen in China (no. 201401049).
Conflicts of interest
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