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Tumor Biology

, Volume 36, Issue 7, pp 5195–5199 | Cite as

The association of GSTM1 and GSTT1 polymorphisms with squamous cell carcinoma of cervix in Pakistan

  • Sarah Hasan
  • Abdul Hameed
  • Saima Saleem
  • S. M. Shahid
  • Ghulam Haider
  • Abid Azhar
Research Article
  • 122 Downloads

Abstract

Gene deletions in GSTM1 and GSTT1 may result in tempering the activation and detoxification of several carcinogens and thereby may increase the risk of cancer pre-disposition. This study aims to investigate the clinical impact of glutathione-S-transferase GSTM1 and GSTT1 polymorphisms on squamous cell carcinoma of cervix (SCCA).The GSTM1 and GSTT1 polymorphisms were analyzed in cervical cancer patients and healthy controls. Touch down multiplex polymerase chain reaction (PCR) strategy was adopted for genotyping of GSTM1 and GSTT1 polymorphisms. The null genotype of GSTM1 exhibited a significantly higher percentage in patients with SCCA (74 %) than in the control group (34.0 %). However, no significant difference was observed in the null genotype of GSTT1 among SCC patients and healthy subjects, respectively. GSTM1 exhibited a significant association with increased risk of squamous cell carcinoma (p < 0.001). The odds ratio for the GSTM1 null genotype was also calculated (odds ratio 3.7484; 95 % confidence interval 1.6562-84834). This suggests that GSTM1 null genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix.

Keywords

Cervical cancer GSTM1 GSTT1 Pakistan Multiplex PCR Genetic polymorphism 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Sarah Hasan
    • 1
  • Abdul Hameed
    • 2
  • Saima Saleem
    • 1
  • S. M. Shahid
    • 1
  • Ghulam Haider
    • 3
  • Abid Azhar
    • 1
  1. 1.The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE)University of KarachiKarachiPakistan
  2. 2.Institute of Biomedical and Genetic Engineering (IB&GE)IslamabadPakistan
  3. 3.Jinnah Post Graduate Medical CollegeKarachiPakistan

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