The association of GSTM1 and GSTT1 polymorphisms with squamous cell carcinoma of cervix in Pakistan
- 122 Downloads
Gene deletions in GSTM1 and GSTT1 may result in tempering the activation and detoxification of several carcinogens and thereby may increase the risk of cancer pre-disposition. This study aims to investigate the clinical impact of glutathione-S-transferase GSTM1 and GSTT1 polymorphisms on squamous cell carcinoma of cervix (SCCA).The GSTM1 and GSTT1 polymorphisms were analyzed in cervical cancer patients and healthy controls. Touch down multiplex polymerase chain reaction (PCR) strategy was adopted for genotyping of GSTM1 and GSTT1 polymorphisms. The null genotype of GSTM1 exhibited a significantly higher percentage in patients with SCCA (74 %) than in the control group (34.0 %). However, no significant difference was observed in the null genotype of GSTT1 among SCC patients and healthy subjects, respectively. GSTM1 exhibited a significant association with increased risk of squamous cell carcinoma (p < 0.001). The odds ratio for the GSTM1 null genotype was also calculated (odds ratio 3.7484; 95 % confidence interval 1.6562-84834). This suggests that GSTM1 null genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix.
KeywordsCervical cancer GSTM1 GSTT1 Pakistan Multiplex PCR Genetic polymorphism
Conflicts of interest
- 3.Raymond MS, Proper S. Squamous cell carcinoma: a review of etiology, pathogenesis, treatment, and variants. J Dermatol Nurs Assoc. 2010;2:16–8.Google Scholar
- 4.Rebbeck TR. Molecular epidemiology of the human glutathione S transferase genotype GSTM1 and GSTT1 in cancer susceptibility. Cancer Epidemiol Biomark Prev. 1997;6:733–43.Google Scholar
- 6.Stewart VN, Vaughan TL, Burt RD, Chen C, Berwick M, Swanson GM. Glutathione S-transferase M1 and susceptibility to nasopharyngeal carcinoma. Cancer Epidemiol Biomarkers Prev. 1999;8:547–51.Google Scholar
- 18.Suzen S, Halit SS, Gulçin G, Mehmet T, Ela C, Yalçin D, et al. The Role of GSTM1 and GSTT1 polymorphisms in head and neck cancer risk. Oncol Res. 2007;6:423–9.Google Scholar
- 21.Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. New York: Cold Spring Harbor Laboratory; 1987.Google Scholar
- 22.Sambrook J, Russel DW. Molecular cloning: a laboratory manual. 3rd ed. New York: Cold Spring Harbor Laboratory; 2001.Google Scholar
- 24.MedCalc. Easy to use statistical software. http://www.medcalc.org/calc/odds_ratio.php. Accessed 01 Jul 2014.