Tumor Biology

, Volume 36, Issue 6, pp 4565–4573 | Cite as

Combined Runx2 and Snail overexpression is associated with a poor prognosis in breast cancer

  • Zhengjun Yang
  • Bin ZhangEmail author
  • Bowen Liu
  • Yegong Xie
  • Xuchen Cao
Research Article


The purpose of this study was to investigate the prognostic value of Runx2 and Snail expression in breast cancer. The expression of Runx2 and Snail in clinical specimens from 125 breast cancer patients was detected by immunohistochemistry. The results showed there is a link between Runx2 and Snail expression at protein levels (p = 0.007). The Kaplan-Meier survival analysis showed that Runx2 or Snail expression was correlated with shortened disease-free survival (DFS) (p = 0.002, p = 0.004, respectively) and overall survival (OS) (p = 0.002, p = 0.009, respectively). In addition, Runx2-positive/Snail-positive patients had the worst DFS and OS (p = 0.001, p < 0.001, respectively). In multivariate survival analysis, Runx2, Snail, and combined Runx2/Snail were still remained as independent prognostic factors for DFS (p = 0.020, p = 0.013, and p = 0.001, respectively) and OS (p = 0.027, p = 0.030 and p = 0.005, respectively). These results suggest that a combined Runx2/Snail expression could be used as a new significant prognostic biomarker for patients with breast cancer.


Breast cancer Prognosis Runx2 Snail EMT 



This research was funded by the National Natural Science Foundation of China (Grant no. 81372843) and the Tianjin Municipal Nature Science Foundation (Grant no. 11JCZDJC28000, 13JCYBJC21800).

Conflicts of interest


Supplementary material

13277_2015_3101_MOESM1_ESM.pdf (172 kb)
ESM 1 (PDF 172 kb)


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Zhengjun Yang
    • 1
    • 2
    • 3
    • 4
  • Bin Zhang
    • 1
    • 2
    • 3
    • 4
    Email author
  • Bowen Liu
    • 1
    • 2
    • 3
    • 4
  • Yegong Xie
    • 1
    • 2
    • 3
    • 4
  • Xuchen Cao
    • 1
    • 2
    • 3
    • 4
  1. 1.Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerTianjinChina
  2. 2.Key Laboratory of Cancer Prevention and TherapyTianjinChina
  3. 3.Key Laboratory of Breast Cancer Prevention and TherapyTianjin Medical University, Ministry of EducationTianjinChina
  4. 4.The First Department of Breast CancerTianjin Medical University Cancer Institute and HospitalTianjinChina

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