Over-expression of miR-10b in NPC patients: correlation with LMP1 and Twist1
- 278 Downloads
Aberrant expression of miR-10b has been described in many cancers but remains unexplored in nasopharyngeal carcinoma (NPC). Therefore, we aimed to study the miR-10b expression level in 43 NPC biopsies collected from Tunisian patients and three NPC xenografts. Then, we investigated the correlation between miR-10b expression and its upstream regulators LMP1/Twist1 as well as its adjacent gene HoxD4. We showed that miR-10b was significantly up-regulated in NPC biopsies compared to non-tumor nasopharyngeal tissues (fold change 153; p = 0.004) and associated with advanced clinical stage and young age at diagnosis (p = 0.005 and p = 0.011, respectively). In addition, over-expression of miR-10b was positively associated with the transcription factor Twist1 as well as the EBV oncoprotein LMP1 (fold change 6.32; p = 0.014, fold change 6.58; p = 0.01 respectively). Furthermore, higher level of miR-10b was observed in tumors with simultaneous expression of LMP1 and Twist1, compared to those expressing only Twist1 (fold change 2.49; p = 0.033). Meanwhile, the analysis of the link between miR-10b and its neighbor gene HoxD4 did not show any significant correlation (Fisher test p = 0.205; Mann–Whitney test p = 0.676). This study reports the first evidence of miR-10b over-expression in NPC patients. Furthermore, our findings can support hsa-miR-10b gene regulation through LMP1/Twist1 in NPC malignancy.
KeywordsNPC miR-10b LMP1 Twist1 Juvenile form
This work was supported by a grant of the Tunisian Ministry of High Education and Scientific Research. We wish to thank Pr Adnane Hammami for allowing us to perform the RT-q-PCR experiments in the microbiology laboratory at the School of Medicine of University of Sfax.
Conflicts of interest
- 18.Yang GD, Huang TJ, Peng LX, Yang CF, Liu RY, Huang HB, et al. Epstein–Barr Virus_Encoded LMP1 upregulates microRNA-21 to promote the resistance of nasopharyngeal carcinoma cells to cisplatin-induced apoptosis by suppressing PDCD4 and Fas-L. Plos One. 2013;8(10):e78355.CrossRefPubMedPubMedCentralGoogle Scholar
- 27.Fleming ID, Cooper JS, Henson DE, Hutter RV, Kennedy BJ, et al. American Joint Committee on Cancer “cancer staging manual”. 5th ed. Philadelphia: Lippincott-Raven; 1997. p. pp. 31–9.Google Scholar
- 28.Sobin LH and Wittekind CH, Eds. International Union Against Cancer. TNM classification of malignant tumours. Wiley-Liss, New York, 5th edition, 1997. pp. 25–32.Google Scholar
- 29.Chan JKC, Pilch BZ, Kuo TT, Wenig BM, Lee AWM. Tumors of the nasopharynx: introduction. In: Barnes L, Eveson JW, Reichart P, Sidransky D, editors. Pathology and genetics of head and neck tumours (World Health Organization classification of tumours). Lyon: IARC; 2005. p. 82–4.Google Scholar