Tumor Biology

, Volume 36, Issue 4, pp 3093–3100 | Cite as

Upregulated expression of miR-106a by DNA hypomethylation plays an oncogenic role in hepatocellular carcinoma

  • Renshun Yuan
  • Qiaoming Zhi
  • Hong Zhao
  • Ye Han
  • Ling Gao
  • Bin Wang
  • Zhongyang Kou
  • Zhaoji Guo
  • Songbing He
  • Xiaofeng Xue
  • Hao Hu
Research Article

Abstract

Aberrant microRNA (miRNA) expression has been widely recognized to play an extremely important role in several cancers, including hepatocellular carcinoma (HCC). According to the previous studies, abnormal miR-106a expression was closely related to various cancer occurrences. However, the miR-106a expression in HCC remains unclear. In our study, we firstly detected the miR-106a expression levels in 36 pairs of HCC tissues. The results showed that miR-106a expression in HCC tissues was apparently higher than the level in the adjacent tissues. Then, we used quantitative real-time PCR (qPCR) and BSP to analyze miR-106a expression and promoter methylation in HCC cell lines. There came to a conclusion that the methylation status of the miR-106a promoter region was inversely correlated with the expression of miR-106a. After prediction with online software, we further used dual-luciferase reporter gene assay to ensure that TP53INP1 and CDKN1A might be the direct targets of miR-106a. At last, we explored the functions of miR-106a in HCC cells in vitro. Our results manifested that high-miR-106a cell line had stronger invasiveness, faster cell cycle progression, and more resistance to apoptosis compared with the low-miR-106a cell line. Therefore, our study suggested that upregulated expression of miR-106a by its promoter hypomethylation might contribute to the progression of HCC, which might be considered as a potentially effective biomarker and therapeutic approach in the future.

Keywords

miR-106a Hypomethylation Oncogene Hepatocellular carcinoma 

Notes

Acknowledgments

This study was supported by a grant from the National Youthful Science Foundation of China (Nos. 81302145 and 81302147) and the National Science Foundation of Jiangsu Province, China (Nos. BK20130268 and 20130270).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Renshun Yuan
    • 1
  • Qiaoming Zhi
    • 1
  • Hong Zhao
    • 1
  • Ye Han
    • 1
  • Ling Gao
    • 1
  • Bin Wang
    • 1
  • Zhongyang Kou
    • 1
  • Zhaoji Guo
    • 1
  • Songbing He
    • 1
  • Xiaofeng Xue
    • 1
  • Hao Hu
    • 1
  1. 1.Department of General SurgeryThe First Affiliated Hospital of Soochow UniversitySuzhouChina

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