Tumor Biology

, Volume 36, Issue 4, pp 2773–2778 | Cite as

RETRACTED ARTICLE: Bone morphogenetic protein 2 mediates epithelial-mesenchymal transition via AKT and ERK signaling pathways in gastric cancer

  • Anyan Liao
  • Weijie Wang
  • Dawei Sun
  • Yuliang Jiang
  • Suqing Tian
  • Jinna Li
  • Xiangshan Yang
  • Ranran Shi
Research Article


Although deregulation of bone morphogenetic protein 2 (BMP2) signaling has been linked to various types of cancers, the relationships between abnormal activation of these signaling pathways and tumorigenesis are not clear in gastric cancer. We hypothesized that BMP2 might be involved in epithelial-mesenchymal transition (EMT) process of gastric cancer. Here, BMPR-II activation and inhibition in gastric cancer cell line AGS were induced with exogenous BMP2 and with BMPR-II small interfering RNA (siRNA), respectively. BMPR-II downstream signal molecules AKT, ERK phosphorylation, and EMT biomarkers (vimentin, snail, N-cadherin, and E-cadherin) were tested using the Western blot. In the present study, our results showed that BMP2 can induce AKT and ERK phosphorylation in a dose-dependent method, and endogenous BMPR-II can be inhibited completely by BMPR-II siRNA in AGS. Notably BMP2 alone treatment can induce the up-regulation of vimentin, snail, and N-cadherin in AGS cells, besides, the down-regulation of E-cadherin also occurred. On the contrary, BMPR-II siRNA significantly prohibited BMP2-induced AKT and ERK phosphorylation, at the same time, EMT biomarkers changes were not observed. On the other hand, BMPR-II knockdown could significantly affect AGS wound closure and the migration ability (p < 0.001) compared to control siRNA and BMP2 alone. In conclusion, this study suggested that EMT process can be triggered by the BMP2/BMPR axis in gastric cancer and then involved in the tumor cell migration, invasion, and metastasis via the activation of PI3K/AKT and MEK/ERK pathways. Our study lays a new foundation for the treatment of gastric cancer through antagonizing BMP2 system.


BMP2 EMT AKT ERK Gastric cancer 



We greatly thank other members in Yang & Sandy Lab for the valuable suggestions and writing.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Anyan Liao
    • 1
  • Weijie Wang
    • 2
  • Dawei Sun
    • 3
  • Yuliang Jiang
    • 1
  • Suqing Tian
    • 1
  • Jinna Li
    • 1
  • Xiangshan Yang
    • 4
  • Ranran Shi
    • 5
  1. 1.Department of Radiation OncologyPeking University 3rd HospitalBeijing,China
  2. 2.Department of Emergency SurgeryQilu Hospital of Shandong UniversityJinanChina
  3. 3.Department of Hepatobiliary and Pancreatic SurgeryThe First Hospital of Jilin UniversityChangchunChina
  4. 4.Department of PathologyAffiliated Hospital of Shandong Academy of Medical SciencesJinanChina
  5. 5.Department of General SurgeryShandong Provincial Hospital Affiliated to Shandong UniversityJinanChina

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