miR-103/107 modulates multidrug resistance in human gastric carcinoma by downregulating Cav-1
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MicroRNAs (miRNAs) are a class of non-protein-coding small RNAs with the capacity to regulate fundamental biological processes essential for cancer initiation and progression. In the present study, we analyzed miRNA expression levels between multidrug-resistant gastric carcinoma cell line SGC7901/ADR and its parent cell line SGC7901 using a miRNA microarray. MiR-103/107 was downregulated compared with parental SGC7901 cells. Overexpression of miR-103/107 sensitized SGC7901/ADR cells to doxorubicin (DOX), as demonstrated by in vitro and in vivo drug sensitivity assay. We further confirmed that miR-103/107 inhibited P-gp function in gastric cancer SGC7901/ADR cells. Finally, we verified that caveolin-1 (Cav-1), a critical component of lipid rafts, was a target of miR-103/107.
KeywordsGastric carcinoma miR-103/107 Multidrug resistance Caveolin-1
This work was supported by the National Science and Technology Major Project (grant no. 2013ZX09303002), the Chinese National Foundation of National Sciences (grant nos. 81270036, 81372485, 81372546, and 81472193), the Science and Technology project of Liaoning Province (grant no. 2013021057), and the Colleges and Universities of Liaoning Province Outstanding Talent Support Plan (grant no. LR2014023).
Conflicts of interest