Tumor Biology

, Volume 36, Issue 2, pp 921–928 | Cite as

Mir-152 inhibits cell proliferation and colony formation of CD133+ liver cancer stem cells by targeting KIT

Research Article

Abstract

miR152 is involved in diverse biological functions and development of disease. This study investigates the role of mir-152 in cell proliferation and colony formation of liver cancer stem cells. We show that exogenous overexpression of mir-152 suppresses cell proliferation and colony formation in CD133+ hep3B cells. We also show that KIT is a direct target of miR-152 and miR-152 downregulates protein expression of KIT by directly binding to 3′ untranslated region of KIT. Downregulation of KIT by specific siRNAs inhibits proliferation and colony formation of CD133+ hep3B cells, which is similar to inhibitory effects of miR-152. Moreover, exogenous expression of KIT compromises inhibitory effects of miR-152 on cell proliferation and colony formation. Our findings suggest that mir-152 inhibits cell proliferation and colony formation of CD133+ hep3B cells by targeting KIT.

Keywords

miR-152 KIT Liver cancer stem cells Proliferation 

Notes

Acknowledgments

The present study was supported by grants from the Science and Technology Program of Guangdong Province (2008B030301028) and the Science and Technology Innovation Fund of Guangdong Medical College (STI F201107).

Conflicts of interest

None

Supplementary material

13277_2014_2719_MOESM1_ESM.docx (18 kb)
ESM 1(DOCX 18 kb)
13277_2014_2719_MOESM2_ESM.doc (28 kb)
Table S1(DOC 28 kb)
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Figure S1(DOCX 62 kb)
13277_2014_2719_MOESM4_ESM.docx (277 kb)
Figure S2(DOCX 277 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Haili Huang
    • 1
  • Min Hu
    • 2
  • Peng Li
    • 1
  • Caijie Lu
    • 2
  • Mingyi Li
    • 2
  1. 1.Clinical Research Centerthe Affiliated Hospital of Guangdong Medical CollegeZhanjiangChina
  2. 2.Department of Hepatobiliary Surgerythe Affiliated Hospital of Guangdong Medical CollegeZhanjiangChina

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