Tumor Biology

, Volume 35, Issue 12, pp 12593–12599 | Cite as

MiR-451 increases radiosensitivity of nasopharyngeal carcinoma cells by targeting ras-related protein 14 (RAB14)

  • Tian Zhang
  • Quanquan Sun
  • Tongxin Liu
  • Jiarong Chen
  • Shasha Du
  • Chen Ren
  • Guixiang Liao
  • Yawei Yuan
Research Article

Abstract

Radioresistance severely impedes the treatment of nasopharyngeal carcinoma (NPC). Recent evidence has shown that the abnormal expression of microRNAs (miRNAs) contributes to radiosensitivity. The aim of this study, therefore, was to investigate whether expression of the miRNAs correlated with radiosensitivity in the context of NPC. Quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to quantify miR-451 expression in two representative NPC cell lines. The role of miR-451 in NPC radiosensitivity was analyzed using a colony formation assay and an immunofluorescence assay with overexpression of miR-451 in NPC cells. Luciferase reporter assays, RT-PCR, and Western blot were performed to confirm the target of miR-451. High levels of miR-451 expression enhanced radiosensitivity in NPC cells by inhibiting the repair of irradiation-induced double-strand breaks (DSBs) and increasing apoptosis. The results also demonstrated that miR-451 directly targeted ras-related protein 14 (RAB14). Downregulation of RAB14 partially replicated the miR-451-mediated DSBs induced by ionizing radiation (IR). MiR-451 could be a potential target for enhancing radiosensitivity of NPC cells by targeting RAB14.

Keywords

MicroRNA MiR-451 RAB14 Nasopharyngeal carcinoma Radiosensitivity 

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Tian Zhang
    • 1
  • Quanquan Sun
    • 1
  • Tongxin Liu
    • 1
  • Jiarong Chen
    • 1
  • Shasha Du
    • 1
  • Chen Ren
    • 1
  • Guixiang Liao
    • 1
  • Yawei Yuan
    • 1
  1. 1.Department of Radiation Oncology, Nanfang HospitalSouthern Medical UniversityGuangzhouPeople’s Republic of China

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