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Tumor Biology

, Volume 35, Issue 11, pp 11137–11145 | Cite as

MicroRNA-486-5p targeting PIM-1 suppresses cell proliferation in breast cancer cells

  • Guoqiang Zhang
  • Zengyan Liu
  • Guanghe Cui
  • Xiaohong Wang
  • Zhenlin Yang
Research Article

Abstract

MicroRNAs (miRNAs) are emerging as critical regulators in carcinogenesis and tumor progression. Recently, miR-486-5p has been proved to play an important role in several cancers, but its functions in the context of breast cancer (BC) remain unknown. In this study, we found that miR-486-5p expression is significantly downregulated in BC tissues and cell lines. Overexpression of miR-486-5p dramatically suppressed BC cell proliferation in vitro and in vivo, induced G0/G1 arrest, and promoted apoptosis. We subsequently identified the oncogene PIM-1 as a direct target of miR-486-5p in BC. Overexpression of PIM-1 attenuated the function of miR-486-5p in BC cells. Together, we conclude that miR-486-5p exerts its antiproliferative function by directly downregulating PIM-1 expression. This novel miR-486-5p/PIM-1 axis provides insight into the pathogenesis of BC and might be therapeutic targets for prevention or treatment of BC.

Keywords

miR-486-5p PIM-1 Breast cancer Proliferation 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (nos. 30973932 and 81173601) and Binzhou Science and Technology Development Plan (no. 2013ZC1708).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Guoqiang Zhang
    • 1
  • Zengyan Liu
    • 2
  • Guanghe Cui
    • 3
  • Xiaohong Wang
    • 1
  • Zhenlin Yang
    • 1
  1. 1.Department of Thyroid and Breast SurgeryAffiliated Hospital of Binzhou Medical CollegeBinzhouChina
  2. 2.Department of HematologyAffiliated Hospital of Binzhou Medical CollegeBinzhouChina
  3. 3.Department of Ultrasonic MedicineAffiliated Hospital of Binzhou Medical CollegeBinzhouChina

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