Tumor Biology

, Volume 35, Issue 11, pp 11013–11019 | Cite as

RETRACTED ARTICLE: MicroRNA-92 promotes gastric cancer cell proliferation and invasion through targeting FXR

Research Article

Abstract

MicroRNAs (miRNAs), a class of small noncoding RNAs, play critical roles in human carcinogenesis through downregulation of various target genes. In the present study, we found that miR-92 is upregulated in gastric cancer tissues compared with adjacent normal tissues. Interestingly, miR-92 expression is significantly associated with clinical characteristics of patients. Gain or loss-of-function in vitro experiments further show that miR-92 mimics significantly promoted, while its antisense oligos inhibited gastric cancer cell proliferation and invasion. Moreover, luciferase reporter assays and western blot indicated that farnesoid X receptor (FXR), is a direct target of miR-92. Therefore, our data suggest that upregulation of miR-92 may represent an important mechanism for the development of gastric cancer.

Keywords

miR-92 Farnesoid X receptor Gastric cancer Invasion 

Notes

Conflict of interest

None

Supplementary material

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Supplementary Figure 1

a Expression levels of miR-92 after transfection of miR-92 mimics or negative controls (NC) in NCI-N87 cells for 24 h. b, c The proliferative potential (BrdU) (b) and invasion assays (c) were determined in NCI-N87 cells transfected with miR-92 mimics or negative controls (NC) for 24 h. (d) The cell cycle phase of NCI-N87 cells transfected with miR-92 mimics or negative controls (NC) were analyzed by flow cytometry. (GIF 4 kb)

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Supplementary Figure 2

Representative FXR protein levels in human gastric cancer tissues and adjacent normal tissues from three patients. (GIF 2 kb)

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High resolution image (TIFF 198 kb)
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Supplementary Figure 3

a, b Relative mRNA (a) and protein (b) levels of C-myc and cyclin D1 were determined by real-time PCR and western blot in AGS cells after transfection of miR-92 mimics or negative controls (NC). (GIF 3 kb)

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Supplementary Figure 4

a, b Relative mRNA (a) and protein (b) levels of C-myc and cyclin D1 were determined by real-time PCR and western blot in AGS cells after transfection of miR-92 antisense or negative controls (NC). (GIF 2 kb)

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High resolution image (TIFF 220 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  1. 1.Department of GastroenterologySecond Hospital of Shaoxing CityShaoxingChina
  2. 2.Department of GastroenterologyChina–Japan Friendship HospitalBeijingChina

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