Tumor Biology

, Volume 35, Issue 11, pp 10855–10860 | Cite as

HOXD10 expression in human breast cancer

  • N. V. Vardhini
  • P. Jagan Mohan Rao
  • P. Balakrishna Murthy
  • G. Sudhakar
Research Article
First Online:
Received:
Accepted:

Abstract

Breast cancer is the most frequent malignancy among females. In this study, we analyzed the expression pattern of a homeobox gene (HOXD10) in human invasive ductal breast cancer tissues and normal tissues. With the ACTB (β-actin) gene as a reference, HOXD10 was detected in 60 breast cancer tissues by using the quantitative real-time PCR (qPCR) method with the Relative Expression Software Tool (REST). We found that the HOXD10 expression level was significantly different between cancerous and normal tissues. Downregulation of the HOXD10 gene expression was examined in high-grade samples. Low-grade tissue showed no difference from the control group. HOXD10 expression was reduced in grade II breast carcinoma tissues. This data reveal that misexpression of the HOXD10 gene supports the development and involvement in breast cancer and may serve as a potential biomarker for the diagnosis of human ductal invasive breast carcinoma.

Keywords

Human breast cancer Gene expression HOXD10 gene Biomarker Quantitative real-time PCR 
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Notes

Acknowledgments

We express our gratitude to Dr. A. Sundaram, Director (retd.), Institute of Pathology, MMC, Chennai, India, for issuing tissue blocks for the study. We thank the management of IIBAT (International Institute of Biotechnology and Toxicology, Padappai, 601 301, Kancheepuram District, Tamil Nadu, India) for their continuous funding and support throughout the study period.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • N. V. Vardhini
    • 1
  • P. Jagan Mohan Rao
    • 1
  • P. Balakrishna Murthy
    • 1
  • G. Sudhakar
    • 2
  1. 1.Department of Genetic ToxicologyInternational Institute of Biotechnology and Toxicology (IIBAT)PadappaiIndia
  2. 2.Department of Human GeneticsAndhra UniversityVisakhapatnamIndia

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