Tumor Biology

, Volume 35, Issue 9, pp 8991–8998 | Cite as

In vitro antileukaemic activity of extracts from Daphne gnidium leaves against sensitive and multidrug resistant K562/R7 cells

  • Fadwa Chaabane
  • Mounira Krifa
  • Eva Matera
  • Amira Loussaeif
  • Marie-Geneviève Dijoux-Franca
  • Kamel Ghedira
  • Charles Dumontet
  • Leila Chekir-Ghedira
Research Article

Abstract

The antiproliferative potential of extracts of Daphne gnidium L. (Thymelaeaceae) on K562 cells was assessed, and the capacity of these extracts to disturb the cell cycle of K562 cells and to inhibit human P-glycoprotein was evaluated. The antiproliferative activity was evaluated using the MTT assay. The cell cycle analysis and the inhibition of P-glycoprotein were tested by flow cytometry. All the tested extracts exhibited significant anti-proliferative effects. Ethyl acetate extract has the strongest cytotoxic effect with an IC50 of 18.5 μg/ml. Furthermore, cell cycle analysis revealed that cells treated with chloroform, butanol and aqueous extracts were arrested predominantly in G2-M phase. Butanol extract was the most active extract. Percentage of cells arrested in G2-M was 34 %, 36.67 % and 42.63 % respectively, after treatment with 25, 75 and 100 μg/ml of the extract, versus 19 % in the cells treated with the vehicle solvent. In addition, chloroform extract had the ability to inhibit human P-glycoprotein-mediated daunorubicin in K562/R7 leukaemic cells in a dose-dependent manner compared to the positive control, cyclosporin A. These findings demonstrate that extracts from D. gnidium leaves have antileukaemic activity by perturbing the cell cycle of K562 and inhibiting human P-glycoprotein in K562/R7 cell line.

Keywords

Daphne gnidium Modulation of antitumour drug resistance Anticancer therapy Polyphenols 

Notes

Acknowledgments

Part of this work was conducted at the University of Lyon, thanks to a scholarship of the Region Rhone-Alpes (MIRA scholarship).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Fadwa Chaabane
    • 1
  • Mounira Krifa
    • 1
  • Eva Matera
    • 2
  • Amira Loussaeif
    • 1
  • Marie-Geneviève Dijoux-Franca
    • 3
  • Kamel Ghedira
    • 1
  • Charles Dumontet
    • 2
  • Leila Chekir-Ghedira
    • 1
    • 4
    • 5
  1. 1.Unité de Substances Naturelles Bioactives et Biotechnologie UR12ES12, Faculté de Pharmacie de MonastirUniversité de MonastirMonastirTunisie
  2. 2.Laboratoire de Cytologie Analytique (Inserm U590)Faculté de MédecineLyon cedex 08France
  3. 3.Equipe Multirésistance Environnementale et Efflux Bactérien, Centre d’Etude des Substances NaturellesUMR 5557 CNRS/Université Lyon1LyonFrance
  4. 4.Laboratoire de Biologie Cellulaire et MoléculaireFaculté de Médecine DentaireMonastirTunisie
  5. 5.Faculté de Médecine dentaireMonastirTunisie

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