Tumor Biology

, Volume 35, Issue 8, pp 7733–7741 | Cite as

Triple-negative and luminal A breast tumors: differential expression of miR-18a-5p, miR-17-5p, and miR-20a-5p

  • Carlos Marino Cabral Calvano Filho
  • Daniele Carvalho Calvano-MendesEmail author
  • Kátia Cândido Carvalho
  • Gustavo Arantes Maciel
  • Marcos Desidério Ricci
  • Ana Paula Torres
  • José Roberto Filassi
  • Edmund Chada Baracat
Research Article


New concepts in epigenetics, microRNAs, and gene expression analysis have significantly enhanced knowledge of cancer pathogenesis over the last decade. MicroRNAs (miRNAs) are a class of non-coding RNAs that regulate gene expression by base pairing with target messenger RNAs (mRNAs), resulting in the repression of translation or the degradation of mRNA. To compare the carcinogenic process in tumors with different prognoses, we used real-time RT-PCR to evaluate the miRNA expression profiles of 24 triple-negative breast invasive ductal carcinoma, 20 luminal A breast invasive ductal carcinoma, and 13 normal breast parenchyma controls. We extracted total RNA from tissues fixed in formol and embedded in paraffin (FFPE). Results revealed the upregulation of miR-96-5p (9.35-fold; p = 0.000115), miR-182-5p (7.75-fold; p = 0.000033), miR-7-5p (6.71-fold; p = 0.015626), and miR-21-5p (6.10-fold; p = 0.000000) in tumors group. In addition, the expression of miR-125b-5p (4.49-fold; p = 0.000000) and miR-205-5p (4.36-fold; p = 0.006098) was downregulated. When the expression profiles of triple-negative and luminal A tumors were compared, there was enhanced expression of miR-17-5p (4.27-fold; p = 0.000664), miR-18a-5p (9.68-fold; p = 0.000545), and miR-20a-5 (4.07-fold; p = 0.001487) in the triple-negative tumors compared with luminal A. These data suggest that there is a similar regulation of certain miRNAs in triple-negative and luminal A tumors. However, it is possible that differences in the expression of miR-17-92 cluster will explain the phenotypic differences between these molecular tumor subtypes.


MicroRNA Breast cancer FFPE tissue Triple-negative Luminal A Cluster miR-17-92 



This research was funded by Fundação de Amparo à Pesquisa do Estado de São paulo—FAPESP, São Paulo-SP, Brazil.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Carlos Marino Cabral Calvano Filho
    • 1
    • 2
  • Daniele Carvalho Calvano-Mendes
    • 1
    • 2
    Email author
  • Kátia Cândido Carvalho
    • 1
  • Gustavo Arantes Maciel
    • 1
  • Marcos Desidério Ricci
    • 2
  • Ana Paula Torres
    • 3
  • José Roberto Filassi
    • 2
  • Edmund Chada Baracat
    • 1
    • 2
  1. 1.Laboratório de Ginecologia Molecular e Estrutural—LIM 58, Disciplina de Ginecologia, Faculdade de MedicinaUniversidade de São PauloSão Paulo-SPBrazil
  2. 2.Setor de Mastologia, Disciplina de Ginecologia, Instituto do Câncer do Estado de São Paulo—ICESP, Faculdade de MedicinaUniversidade de São PauloSão Paulo-SPBrazil
  3. 3.Fundação Oncocentro de São PauloSão Paulo-SPBrazil

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