Skip to main content

Advertisement

SpringerLink
Log in

Antitumor activity of a polysaccharide from longan seed on lung cancer cell line A549 in vitro and in vivo

  • Research Article
  • Published:
Tumor Biology

Abstract

In this study, a water-soluble longan seed polysaccharide (WLSP), with a molecular weight of 57 kDa, was isolated from longan seed. Gas chromatography (GC) analysis showed that WLSP was composed mainly of rhamnose (Rha), mannose (Man), arabinose (Ara), galactose (Gal), and glucose (Glc), with molar ratios of 2.4:1.5:2.3:5.6:6.5. The result in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that WLSP showed a dose-dependent antiproliferative effect on the proliferation of A549 human lung cancer cells, which is consistent with the amount of lactate dehydrogenase (LDH) release from A549 cells. Prompted by this antiproliferative effect, we further examined its antiproliferative mechanism and in vivo anticancer effect. Our results showed that WLSP had the ability to cause cell cycle arrest in G1 phase, activation of caspases 3 and 9, and cleavage of poly[ADP (ribose)] polymerase (PARP) in A549 cells. The result of this in vivo study showed that WLSP could suppress the growth of xenograft A549 tumors and induce apoptosis. Taken together, these results indicate that WLSP exert an anticancer effect in vitro and in vivo and may be useful for the prevention of lung tumorigenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Worasuttayangkurn L, Watcharasit P, Rangkadilok N, Suntararuks S, Khamkong P, Satayavivad J. Safety evaluation of longan seed extract: acute and repeated oral administration. Food Chem Toxicol. 2012;50:3949–55.

    Article  CAS  PubMed  Google Scholar 

  2. Jiang YM, Zhang ZQ, Joyce DC, Ketsa S. Postharvest biology and handling of longan fruit (Dimocarpus longan Lour.). Postharvest Biol Technol. 2002;26:241–52.

    Article  Google Scholar 

  3. Xiao G, Huang R, Zeng Q, Chen W, Liu X, Rui H. Nutrition composition of longan seeds. Shi Pin Ke Ji. 2004;1:93–4.

    Google Scholar 

  4. Zheng G, Wei X, Xu L, Li Z, Liu G, Zhang X. A new natural lactone from Dimocarpus longan Lour. seeds. Molecules. 2012;17:9421–5.

    Article  CAS  PubMed  Google Scholar 

  5. Rangkadilok N, Sitthimonchai S, Worasuttayangkurn L, Mahidol C, Ruchirawat M, Satayavivad J. Evaluation of free radical scavenging and antityrosinase activities of standardized longan fruit extract. Food Chem Toxicol. 2007;45:328–36.

    Article  CAS  PubMed  Google Scholar 

  6. Zheng SQ, Jiang F, Gao HY, Zheng JG. Preliminary observations on the antifatigue effects of longan (Dimocarpus longan Lour.) seed polysaccharides. Phytother Res. 2010;24:622–4.

    CAS  PubMed  Google Scholar 

  7. Chung YC, Lin CC, Chou CC, Hsu CP. The effect of longan seed polyphenols on colorectal carcinoma cells. Eur J Clin Invest. 2010;40:713–21.

    Article  CAS  PubMed  Google Scholar 

  8. Huang RQ, Zou XY, Liu XM. Study on the hypoglycemic effect of longan seed extract. Nat Prod Res Dev. 2006;18:991–2.

    Google Scholar 

  9. Soong YY, Barlow PJ. Isolation and structure elucidation of compounds from longan (Dimocarpus longan Lour.) seed by high-performance liquid chromatography-electrospray ionization mass spectrometry. J Chromatogr A. 2005;1085:270–7.

    Article  CAS  PubMed  Google Scholar 

  10. Rangkadilok N, Worauttayngkurn L, Bennett RN, Satayavivad J. Identification and quantification of polyphenolic compounds in longan (Euphoria longana Lam.) fruit. J Agric Food Chem. 2005;53:1387–92.

    Article  CAS  PubMed  Google Scholar 

  11. Minakata H, Komura H, Tamura SY, Ohfune Y, Nakanishi K, Kada T. Anti-mutagenic unusual amino acids from plants. Experientia. 1985;41:1621–2.

    Article  Google Scholar 

  12. Jiang G, Wen L, Chen F, Wu F, Lin S, Yang B, et al. Structural characteristics and antioxidant activities of polysaccharides from longan seed. Carbohydr Polym. 2013;92:758–64.

    Article  CAS  PubMed  Google Scholar 

  13. Wen LR, Lin LZ, You LJ, Yang B, Jiang GX, Zhao MM. Ultrasound-assisted extraction and structural identification of polysaccharides from Isodon lophanthoides var. gerardianus (Bentham) H. Hara. Carbohydr Polym. 2011;85:541–7.

    Article  CAS  Google Scholar 

  14. Staub AM. Removal of protein: Sevag method. Methods Carbohydr Chem. 1965;5:5–6.

    CAS  Google Scholar 

  15. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem. 1956;28:350–6.

    Article  CAS  Google Scholar 

  16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Chem. 1976;72:248–54.

    CAS  Google Scholar 

  17. Blumenkrantz N, Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973;54:484–9.

    Article  CAS  PubMed  Google Scholar 

  18. Luo AX, He XJ, Zhou S, Fan YJ, Luo AS, Chun Z. Purification, composition analysis and antioxidant activity of the polysaccharides from Dendrobium nobile Lindl. Carbohydr Polym. 2010;79:1014–9.

    Article  CAS  Google Scholar 

  19. Zhai Q, Li X, Yang Y, Yu L, Yao Y. Antitumor activity of a polysaccharide fraction from Laminaria japonica on U14 cervical carcinoma-bearing mice. Tumour Biol. 2014;35:117–22.

    Article  CAS  PubMed  Google Scholar 

  20. Lee TH, Chuang LY, Hung WC. Tamoxifen induces p21Waf1 and p27Kip1 expression in estrogen receptor-negative lung cancer cells. Oncogene. 1999;18:4269–74.

    Article  CAS  PubMed  Google Scholar 

  21. Sawaoka H, Tsuji S, Tsujii M, Gunawan ES, Sasaki Y, Kawano S, et al. Cyclooxygenase inhibitors suppress angiogenesis and reduce tumor growth in vivo. Lab Invest. 1999;79:1469–77.

    CAS  PubMed  Google Scholar 

  22. Waheed Roomi M, Ivanov V, Kalinovsky T, Niedzwiecki A, Rath M. In vivo and in vitro antitumor effect of a unique nutrient mixture on lung cancer cell line A-549. Exp Lung Res. 2006;32:441–53.

    Article  CAS  PubMed  Google Scholar 

  23. Yin X, Zhou J, Jie C, Xing D, Zhang Y. Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549. Life Sci. 2004;75:2233–44.

    Article  CAS  PubMed  Google Scholar 

  24. Punathil T, Katiyar SK. Inhibition of non-small cell lung cancer cell migration by grape seed proanthocyanidins is mediated through the inhibition of nitric oxide, guanylate cyclase, and ERK1/2. Mol Carcinog. 2009;48:232–42.

    Article  CAS  PubMed  Google Scholar 

  25. Choi WH, Chu JP, Jiang MH, Baek SH, Park HD. Effects of fraction obtained from Korean Corni Fructus extracts causing anti-proliferation and p53-dependent apoptosis in A549 lung cancer cells. Nutr Cancer. 2011;63:121–9.

    PubMed  Google Scholar 

  26. Houghton PJ. The role of plants in traditional medicine and current therapy. J Altern Complement Med. 1995;1:131–43.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31100656).

Conflicts of interest

None

Author information

Authors and Affiliations

  1. Department of Respiratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China

    Hongmin Wang, Yuexia Li, Ruiying Chen, Songyun Ouyang & Peizong Sun

  2. Department of Respiratory, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, 450053, China

    Xiaohong Zhang

  3. Department of Pulmonary Diseases, Tangdu Hospital, The Fourth Military Medical University, Xi’an, 710038, China

    Lei Pan

  4. Department of Thoracic Surgery, The First Affiliated Hospital of Health Science Center of Xi’an Jiao Tong University, Xi’an, 710061, China

    Hong Ren & Bo Yang

Authors
  1. Hongmin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaohong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yuexia Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ruiying Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Songyun Ouyang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Peizong Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lei Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hong Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Bo Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Lei Pan.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, H., Zhang, X., Li, Y. et al. Antitumor activity of a polysaccharide from longan seed on lung cancer cell line A549 in vitro and in vivo. Tumor Biol. 35, 7259–7266 (2014). https://doi.org/10.1007/s13277-014-1927-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13277-014-1927-8

Keywords

Search

Navigation