Tumor Biology

, Volume 35, Issue 6, pp 6047–6054 | Cite as

Thrombomodulin mediates the migratory ability of hormone-independent prostate cancer cells through the regulation of epithelial-to-mesenchymal transition biomarkers

  • Chun-Te Wu
  • Yu-Jia Chang
  • Miao-Fen Chen
  • Jun-Jen Liu
  • Po-Li Wei
  • Weu WangEmail author
  • Hui-Hsiung LiuEmail author
Research Article


Thrombomodulin (TM) is highly expressed in endothelial cells and plays the key role in maintaining physical homeostasis. In addition, many pieces of evidence also show that TM contains the diagnostic value for malignant diseases. TM has been found to correlate with metastatic status in multiple cancers, but its role in prostate cancer progression remains unclear. TM expression was determined in prostate cancer cells (DU-145 and PC-3 cells) using real-time PCR and Western blotting. TM expression was manipulated in prostate cancer cells using TM-specific shRNA and an overexpression system. The proliferation, adhesion, and migratory ability of prostate cancer cells expressing various TM levels were determined using the x’Celligence biosensor system and a transwell migration assay. Higher levels of TM transcription and translation were found in DU-145 cells and were negatively correlated with the low migratory ability of DU-145 cells. After silencing TM expression in DU-145 cells, cell growth decreased, but cell adhesion and migration dramatically increased. TM overexpression in PC-3 cells reduced their metastatic ability. We investigated the possible mechanisms of this phenomenon and determined that the enhanced cell migration was mediated through the expression of E-cadherin and vimentin. TM may be a modulator of hormone-independent prostate cancer (HIPC) metastasis. The downregulation of TM expression enhanced the migratory ability of these cells via an increase in vimentin expression and a decrease in E-cadherin expression.


Thrombomodulin (TM) Prostate cancer Migration Epithelial-to-mesenchymal transition (EMT) 



This study was supported by a grant from Chang Gung Memorial Hospital (CMRPG2B0323) and a grant from Taipei Medical University (TMU100-AE3-Y14).

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Chun-Te Wu
    • 1
    • 2
  • Yu-Jia Chang
    • 3
    • 4
    • 5
    • 6
  • Miao-Fen Chen
    • 2
    • 7
  • Jun-Jen Liu
    • 8
  • Po-Li Wei
    • 3
    • 4
    • 5
    • 6
  • Weu Wang
    • 4
    • 5
    Email author
  • Hui-Hsiung Liu
    • 9
    Email author
  1. 1.Department of UrologyChang Gung Memorial HospitalKeelungTaiwan
  2. 2.Chang Gung University College of Medicine and Chang Gung Institute of TechnologyTao-YuanTaiwan
  3. 3.Graduate Institute of Clinical Medicine, College of MedicineTaipei Medical UniversityTaipeiTaiwan
  4. 4.Department of SurgeryTaipei Medical UniversityTaipeiTaiwan
  5. 5.Division of General SurgeryTaipei Medical University and HospitalTaipeiTaiwan
  6. 6.Cancer Research CenterTaipei Medical University and HospitalTaipeiTaiwan
  7. 7.Department of Radiation OncologyChang Gung Memorial HospitalChiayiTaiwan
  8. 8.School of Medical Laboratory Science and BiotechnologyTaipei Medical UniversityTaipeiTaiwan
  9. 9.Department of Public Health, School of Public HealthTaipei Medical UniversityTaipei CityTaiwan

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