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Tumor Biology

, Volume 35, Issue 6, pp 5409–5415 | Cite as

Oxymatrine triggers apoptosis by regulating Bcl-2 family proteins and activating caspase-3/caspase-9 pathway in human leukemia HL-60 cells

  • Jun Liu
  • Yazhou Yao
  • Huifang Ding
  • Renan ChenEmail author
Research Article

Abstract

With the objective of identifying promising antitumor agents for human leukemia, we carried out to determine the anticancer ability of oxymatrine on the human leukemia HL-60 cell line. In vitro experiments demonstrated that oxymatrine reduced the proliferation of HL-60 cells in a dose- and time-dependent manner via the induction of apoptosis and cell cycle arrest at G2/M and S phases. The proteins involved in oxymatrine-induced apoptosis in HL-60 cells were also examined using Western blot. The increase in apoptosis upon treatment with oxymatrine was correlated with downregulation of anti-apoptotic Bcl-2 expression and upregulation of pro-apoptotic Bax expression. Furthermore, oxymatrine induced the activation of caspase-3 and caspase-9 and the cleavage of poly(ADP-ribose) polymerase (PARP) in HL-60 cells. In addition, pretreatment with a specific caspase-3 (Z-DEVD-FMK) or caspase-9 (Z-LEHD-FMK) inhibitor significantly neutralized the pro-apoptotic activity of oxymatrine in HL-60 cells, demonstrating the important role of caspase-3 and caspase-9 in this process. Taken together, these results indicated that oxymatrine-induced apoptosis may occur through the activation of the caspase-9/caspase-3-mediated intrinsic pathway. Therefore, oxymatrine may be a potential candidate for the treatment of human leukemia.

Keywords

Oxymatrine Apoptosis HL-60 Caspase Bax/Bcl-2 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  1. 1.Department of Geriatrics, Tangdu Hospitalthe Fourth Military Medical UniversityXi’anChina
  2. 2.Department of Haematologic and RheumatismBaoji Central HospitalBaoJiChina
  3. 3.Department of HaematologyShengli Oilfield Central HospitalDongyingChina
  4. 4.Department of Haematology, Tangdu Hospitalthe Fourth Military Medical UniversityXi’anChina

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