Tumor Biology

, Volume 35, Issue 5, pp 5013–5019 | Cite as

RETRACTED ARTICLE: Polyamidoamine dendrimer liposome-mediated survivin antisense oligonucleotide inhibits hepatic cancer cell proliferation by inducing apoptosis

  • Shuai Han
  • Zhai Cai
  • Liang Peng
  • Zhou Li
  • Hua-Bin Zhou
  • Xiu-Qin Li
  • Su-Zhen Fang
  • Zong-Hai Huang
  • Da-Xiang Cui
Research Article

Abstract

Polyamidoamine dendrimer (PAMAM) is a new nanometer material, which can transfer the target genes to cells with high efficiency and lower toxicity. This study aims to evaluate antitumor effects of survivin antisense oligonucleotide (survivin-asODN) (carried by polyamidoamine dendrimer liposome) on hepatic cancer in nude mice. Hepatic cancer model was established by injecting SMMC-7721 cells subcutaneously into flanks of nude mice. Polyamidoamine dendrimer and liposome were mixed with survivin-asODN, respectively. The shape and size of complex were observed by transmission electron microscope, and zeta potential was measured by an analytical tool. Encapsulation efficiency and DNA loading level were determined by an ultraviolet spectrophotometer in centrifuging method. Expression of survivin in transplant tumor was measured by Western blotting. No significant difference appeared for diameter and envelopment ratio between PAMAM liposome-survivin-asODN and PAMAM-survivin-asODN (P > 0.05). Both zeta potential and transfection efficiency in PAMAM liposome-survivin-asODN were higher than that in PAMAM-survivin-asODN complex (P < 0.05). Expression of survivin protein and weight of tumors in transplanted tumors in PAMAM liposome-survivin-asODN group was less than that in PAMAM-survivin-asODN group (P < 0.05). Cell apoptosis rate in PAMAM liposome-survivin-asODN group was higher than that of PAMAM-survivin-asODN group (P < 0.05). In conclusion, polyamidoamine dendrimer liposome can deliver survivin-asODN into hepatic transplanted tumor cells effectively. Ployamidoamine dendrimer liposome-mediated survivin-asODN can inhibit hepatic cell proliferation by inducing apoptosis.

Keywords

Polyamidoamine dendrimer liposome Hepatic cancer Antisense oligonucleotide Survivin 

Notes

Acknowledgement

This research was supported by the Natural Science Foundation of Guangdong Province (no. 9151051501000071).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Shuai Han
    • 1
  • Zhai Cai
    • 1
  • Liang Peng
    • 1
  • Zhou Li
    • 1
  • Hua-Bin Zhou
    • 1
  • Xiu-Qin Li
    • 1
  • Su-Zhen Fang
    • 1
  • Zong-Hai Huang
    • 1
  • Da-Xiang Cui
    • 2
  1. 1.Department of General Surgery, Zhujiang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Research Institute of Micro/Nanometer Science and TechnologyShanghai Jiao Tong UniversityShanghaiChina

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