Tumor Biology

, Volume 35, Issue 5, pp 5013–5019 | Cite as

RETRACTED ARTICLE: Polyamidoamine dendrimer liposome-mediated survivin antisense oligonucleotide inhibits hepatic cancer cell proliferation by inducing apoptosis

  • Shuai Han
  • Zhai Cai
  • Liang Peng
  • Zhou Li
  • Hua-Bin Zhou
  • Xiu-Qin Li
  • Su-Zhen Fang
  • Zong-Hai Huang
  • Da-Xiang Cui
Research Article


Polyamidoamine dendrimer (PAMAM) is a new nanometer material, which can transfer the target genes to cells with high efficiency and lower toxicity. This study aims to evaluate antitumor effects of survivin antisense oligonucleotide (survivin-asODN) (carried by polyamidoamine dendrimer liposome) on hepatic cancer in nude mice. Hepatic cancer model was established by injecting SMMC-7721 cells subcutaneously into flanks of nude mice. Polyamidoamine dendrimer and liposome were mixed with survivin-asODN, respectively. The shape and size of complex were observed by transmission electron microscope, and zeta potential was measured by an analytical tool. Encapsulation efficiency and DNA loading level were determined by an ultraviolet spectrophotometer in centrifuging method. Expression of survivin in transplant tumor was measured by Western blotting. No significant difference appeared for diameter and envelopment ratio between PAMAM liposome-survivin-asODN and PAMAM-survivin-asODN (P > 0.05). Both zeta potential and transfection efficiency in PAMAM liposome-survivin-asODN were higher than that in PAMAM-survivin-asODN complex (P < 0.05). Expression of survivin protein and weight of tumors in transplanted tumors in PAMAM liposome-survivin-asODN group was less than that in PAMAM-survivin-asODN group (P < 0.05). Cell apoptosis rate in PAMAM liposome-survivin-asODN group was higher than that of PAMAM-survivin-asODN group (P < 0.05). In conclusion, polyamidoamine dendrimer liposome can deliver survivin-asODN into hepatic transplanted tumor cells effectively. Ployamidoamine dendrimer liposome-mediated survivin-asODN can inhibit hepatic cell proliferation by inducing apoptosis.


Polyamidoamine dendrimer liposome Hepatic cancer Antisense oligonucleotide Survivin 



This research was supported by the Natural Science Foundation of Guangdong Province (no. 9151051501000071).

Conflicts of interest



  1. 1.
    Yuan L, Zhao H, Zhang L, Liu X. The efficacy of combination therapy using adeno-associated virus-mediated co-expression of apoptin and interleukin-24 on hepatocellular carcinoma. Tumor Biol. 2013;34(5):3027–2034.CrossRefGoogle Scholar
  2. 2.
    Pan B, Cui D, Sheng Y, Ozkan C, Gao F, He R, et al. Dendrimer-modified magnetic nanoparticles enhance efficiency of gene delivery system. Cancer Res. 2007;67(17):8156–63.CrossRefPubMedGoogle Scholar
  3. 3.
    Bhattacharyya S, Kudgus RA, Bhattacharya R, Mukherjee P. Inorganic nanoparticles in cancer therapy. Pharm Res. 2011;28(2):237–59.CrossRefPubMedGoogle Scholar
  4. 4.
    Li Z, Fang SZ, Han S, Cui DX, Li Q, Cai Z, et al. Survivin antisense oligonucleotide mediated by polyamidoaminedendrimer liposome induces apoptosis of hepatic cancer cells. Chin J Cancer Biother. 2012;19(4):409–13.Google Scholar
  5. 5.
    Li XQ, Pei DS, Qian GW, Yin XX, Cheng Q, Li LT, et al. The effect of methylated oligonucleotide targeting Ki-67 gene in human 786-O renal carcinoma cells. Tumor Biol. 2011;32(5):863–72.CrossRefGoogle Scholar
  6. 6.
    Millan A, Huerta S. Apoptosis-inducing factor and colon cancer. J Surg Res. 2009;151(1):163–70.CrossRefPubMedGoogle Scholar
  7. 7.
    Sun NF, Tian AL, Liu ZA, Hu SY, Hu AB. Antiapoptotic gene BAG-1 vector structure of RNA interference and endogenous targeted screening in colon cancer cell lines. Tumor Biol. 2013. doi: 10.1007/s13277-013-1140-1.Google Scholar
  8. 8.
    Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med. 1997;3(8):917–21.CrossRefPubMedGoogle Scholar
  9. 9.
    Zhang Y, Zhang Y, Chen J, Zhang B, Pan Y, Ren L, et al. Polybutylcyanoacrylate nanoparticles as novel vectors in cancer gene therapy. Nanomedicine. 2007;3(2):144–53.CrossRefPubMedGoogle Scholar
  10. 10.
    Ao L, Gao F, Pan B, He R, Cui D. Fluoroimmunoassay for antigen based on fluorescence quenching signal of gold nanoparticles. Anal Chem. 2006;78(4):1104–6.CrossRefPubMedGoogle Scholar
  11. 11.
    Li F, Altieri DC. Transcriptional analysis of human survivin gene expression. Biochem J. 1999;344(2):305–11.PubMedPubMedCentralGoogle Scholar
  12. 12.
    Reed JC. Dysregulation of apoptosis in cancer. J Clin Oncol. 1999;17(9):2941–53.CrossRefPubMedGoogle Scholar
  13. 13.
    Cui D, Huang P, Zhang C, Ozkan CS, Pan B, Xu P. Dendrimer-modified gold nanorods as efficient controlled gene delivery system under near-infrared light irradiation. J Control Release. 2011;152 Suppl 1:e137–9.CrossRefPubMedGoogle Scholar
  14. 14.
    Svenson S. Dendrimers as versatile platform in drug delivery applications. Eur J Pharm Biopharm. 2009;71(3):445–62.CrossRefPubMedGoogle Scholar
  15. 15.
    Hector S, Rehm M, Schmid J, Kehoe J, McCawley N, Dicker P, et al. Clinical application of a systems model of apoptosis execution for the prediction of colorectal cancer therapy responses and personalisation of therapy. Gut. 2012;61(5):725–33.CrossRefPubMedGoogle Scholar
  16. 16.
    Wong PE, Tetley L, Dufes C, Chooi KW, Bolton K, Schatzlein AG, et al. Polyamine aza-cyclic compounds demonstrate anti-proliferative activity in vitro but fail to control tumour growth in vivo. J Pharm Sci. 2010;99(11):4642–57.CrossRefPubMedGoogle Scholar
  17. 17.
    Prabha S, Zhou WZ, Panyam J, Labhaseltwar V. Size-dependency of nanoparticle-mediated gene transfection: studies with fractionated nanoparticles. Int J Pharm. 2002;244(1–2):105–15.CrossRefPubMedGoogle Scholar
  18. 18.
    Navarro G, Tros DIC. Activated and non-activated PAMAM dendrimers for gene delivery in vitro and in vivo. Nanomedicine. 2009;5(3):287–97.CrossRefPubMedGoogle Scholar
  19. 19.
    Huang M, Khor E, Lim LY. Uptake and cytotoxicity of chitosan molecules and nanoparticles: effects of molecular weight and degree of deacetylation. Pharm Res. 2004;21(2):344–53.CrossRefPubMedGoogle Scholar
  20. 20.
    Wang C, Wang W, Wang J, Zhan H, Jiang L, Yan R, et al. Apoptin induces apoptosis in nude mice allograft model of human bladder cancer by altering multiple bladder tumor associated gene expression profiles. Tumor Biol. 2013;34(3):1667–78.CrossRefGoogle Scholar
  21. 21.
    Surendiran A, Sandhiya S, Pradhan SC, Adithan C. Novel applications of nanotechnology in medicine. Indian J Med Res. 2009;130(6):689–701.PubMedGoogle Scholar

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Shuai Han
    • 1
  • Zhai Cai
    • 1
  • Liang Peng
    • 1
  • Zhou Li
    • 1
  • Hua-Bin Zhou
    • 1
  • Xiu-Qin Li
    • 1
  • Su-Zhen Fang
    • 1
  • Zong-Hai Huang
    • 1
  • Da-Xiang Cui
    • 2
  1. 1.Department of General Surgery, Zhujiang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Research Institute of Micro/Nanometer Science and TechnologyShanghai Jiao Tong UniversityShanghaiChina

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