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Tumor Biology

, Volume 34, Issue 6, pp 3995–4000 | Cite as

Prognostic significance of nemo-like kinase (NLK) expression in patients with gallbladder cancer

  • Maolan Li
  • Shenglai Zhang
  • Zhiqiang Wang
  • Bingtai Zhang
  • Xiangsong Wu
  • Hao Weng
  • Qian Ding
  • Zhujun Tan
  • Ning Zhang
  • Jiasheng Mu
  • Jiahua Yang
  • Yijun Shu
  • Runfa Bao
  • Qichen Ding
  • Wenguang Wu
  • Yang Cao
  • Yingbin Liu
Research Article

Abstract

Nemo-like kinase (NLK), a serine/threonine protein kinase, has been implicated in tumor development and progression, and plays an important role in diverse signaling pathways by phosphorylating a variety of transcription factors. Recent studies demonstrated that altered expression of NLK was observed in various types of human cancers. However, the clinical significance of NLK expression in gallbladder cancer (GBC) remains largely unknown. In this study, we focused on the clinical significance of NLK in GBC, and found that nuclear NLK protein overexpression was frequently detected in GBC tissues. The overexpression of NLK was significantly correlated with histological grade, TNM stage, and perineural invasion. The results of Kaplan–Meier analysis indicated that a high expression level of NLK resulted in a significantly poorer prognosis of GBC patients (P = 0.002). Furthermore, multivariate Cox regression analysis showed that high NLK expression was an independent prognostic factor for GBC patients (HR = 3.077). In conclusion, overexpression of NLK is closely related to progression of GBC, and NLK could be used as a potential prognostic marker for GBC patients.

Keywords

Gallbladder cancer Nemo-like kinase Immunohistochemistry 

Notes

Acknowledgments

This work was supported by Xinhua hospital (No. 12YJ01), the Shanghai Committee of Science and Technology (Nos. 12JC1406700, 12410705900, 12401905800), the National Natural Science Foundation of China (Nos. 81172029, 81272402), the Foundation of Shanghai Outstanding Academic Leaders (No.11XD1403800), the National High Technology Research and Development Program (863 Program; No.2012AA022606), and the Medical and Engineering Cross-Foundation of Shanghai Jiaotong University (No. YG2011ZD07).

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Maolan Li
    • 1
    • 2
  • Shenglai Zhang
    • 1
    • 2
  • Zhiqiang Wang
    • 3
  • Bingtai Zhang
    • 4
  • Xiangsong Wu
    • 1
    • 2
  • Hao Weng
    • 1
    • 2
  • Qian Ding
    • 1
    • 2
  • Zhujun Tan
    • 1
    • 2
  • Ning Zhang
    • 4
  • Jiasheng Mu
    • 1
    • 2
  • Jiahua Yang
    • 1
    • 2
  • Yijun Shu
    • 1
    • 2
  • Runfa Bao
    • 1
    • 2
  • Qichen Ding
    • 1
    • 2
  • Wenguang Wu
    • 1
    • 2
  • Yang Cao
    • 1
    • 2
  • Yingbin Liu
    • 1
    • 2
  1. 1.Research Institute of Biliary Tract Disease Affiliated to School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of General Surgery, Xinhua hospital, School of MedicineShanghai Jiaotong UniversityShanghaiPeople’s Republic of China
  3. 3.Department of General SurgeryShanghai Changning HospitalShanghaiPeople’s Republic of China
  4. 4.Department of General SurgeryShanxi Medical University Second HospitalTaiyuanPeople’s Republic of China

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