Tumor Biology

, Volume 34, Issue 3, pp 1919–1923

Interactions of miR-34b/c and TP-53 polymorphisms on the risk of nasopharyngeal carcinoma

Research Article

Abstract

Growing evidence indicates that tumor suppressor gene TP-53 and non-coding RNA miR-34b/c independently and/or jointly play crucial roles in carcinogenesis. We hypothesized that the polymorphisms of rs4938723 in the promoter region of pri-miR-34b/c and TP-53 Arg72-Pro may be related to the risk of nasopharyngeal carcinoma (NPC). We performed a case–control study between 217 patients with NPC and 360 healthy controls in a Chinese population using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) assay. A significantly increased risk of NPC was observed in the miR-34b/c rs4938723 CT/CC genotypes compared with the TT genotype (adjusted OR = 1.44, 95 % CI 1.02–2.03, p = 0.04), and also the C allele (adjusted OR = 1.33, 95 % CI 1.04–1.70, p = 0.03). The gene–gene interaction of miR-34b/c rs4938723 and TP-53 Arg72-Pro showed that the combined genotypes of rs4938723CT/CC and TP-53CG/CC increased the risk of NPC (rs4938723CT/CC + TP-53CG/CC vs. rs4938723 TT + TP-53 CG/CC: OR = 1.58, 95 % CI 1.04–2.42, p = 0.03). These findings suggest that miR-34b/c rs4938723 and TP-53 Arg72Pro polymorphisms may singly or collaboratively contribute to the risk of NPC.

Keywords

Nasopharyngeal carcinoma miR-34b/c TP-53 Genetic polymorphism 

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  1. 1.Department of Forensic Biology, West China School of Preclinical and Forensic MedicineSichuan UniversityChengduPeople’s Republic of China
  2. 2.Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children’s Health, West China Second University HospitalSichuan UniversityChengduPeople’s Republic of China
  3. 3.Department of Head and Neck OncologyChongqing Cancer HospitalChongqingPeople’s Republic of China
  4. 4.Department of Neurosurgery, West China HospitalSichuan UniversityChengduPeople’s Republic of China
  5. 5.Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth DefectsMinistry of EducationChengduPeople’s Republic of China

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