Tumor Biology

, Volume 34, Issue 3, pp 1865–1871 | Cite as

The regulatory mechanism of CCR7 gene expression and its involvement in the metastasis and progression of gastric cancer

  • Wei-ning Wang
  • Yuxiang Chen
  • Yang-de Zhang
  • Tie-hui HuEmail author
Research Article


Gastric cancer is the second leading cause of cancer mortality, but the molecular mechanisms underlying its progression and metastasis remain unclear. CCR7 and Dicer 1 protein expression in 80 gastric adenocarcinomas and 40 peritumoral tissues were measured by immunohistochemical staining. The expression of let-7a miRNA in serum, tumor tissues, and peritumoral tissues was measured by real-time PCR. The role of let-7a in CCR7 protein expression, migration, and invasion of gastric cancer cells was tested in vitro. Dicer 1 protein expression was found to be significantly reduced, whereas CCR7 protein expression was significantly increased in gastric adenocarcinomas compared to peritumoral tissues. The let-7a miRNA levels in the serum and tumor tissues of gastric adenocarcinoma patients were significantly lower than in the serum of healthy controls and peritumoral tissues, respectively. Dicer 1 protein positively correlated with let-7a miRNA level, but negatively correlated with CCR7 protein level in gastric adenocarcinoma. Negative Dicer 1 protein and let-7a miRNA expression and positive CCR7 protein expression significantly correlated with lymph node metastasis, depth of invasion, high clinical TNM stage, and larger tumor size. Let-7a transfection significantly inhibited CCR7 protein expression, migration, and invasion of MNK-45 cells in vitro. High expression of CCR7 protein and low expression of Dicer 1 protein and let-7a miRNA are significantly associated with the metastasis and progression of gastric cancer. High CCR7 protein expression may be caused by the loss of Dicer 1 protein expression and reduced let-7a miRNA level in gastric cancer. The serum let-7a level might be a marker for the diagnosis of gastric cancer.


CCR7 Dicer 1 Let-7a miRNA Gastric cancer Metastasis 



We acknowledge the financial support of the National Hi-tech Program (863 Project) of China (no. 2007AA021804, 2007AA021809).

Conflict of interest

All authors declared no conflict of interest.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2013

Authors and Affiliations

  • Wei-ning Wang
    • 1
  • Yuxiang Chen
    • 1
  • Yang-de Zhang
    • 1
  • Tie-hui Hu
    • 1
    Email author
  1. 1.Hepatobiliary & Enteric Surgery Research Center, Xiangya HospitalCentral South UniversityChangshaChina

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