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Tumor Biology

, Volume 34, Issue 2, pp 629–636 | Cite as

Overexpression of amyloid precursor protein in acute myeloid leukemia enhances extramedullary infiltration by MMP-2

  • Ling Jiang
  • Guopan Yu
  • Wei Meng
  • Zhixiang Wang
  • Fanyi Meng
  • Wenli Ma
Research Article

Abstract

It is known that leukemia patients with extramedullary infiltration (EMI) have a worse prognosis than patients without it. Recent data indicate that the amyloid precursor protein (APP) is involved in cell adhesion, motility, and proliferation. The expression of APP and its prognostic significance in acute myeloid leukemia (AML) have not been studied. Our study shows that AML/ETO+ leukemia patients that overexpress APP easily get EMI and that their long-term survival rate is lower than patients without overexpression of APP. In an in vitro study, we knocked down APP in Kasumi-1 cells using small interfering RNA (siRNA). Transwell data show that siRNA/APP substantially impairs cell migration, but it does not inhibit cell proliferation. Furthermore, by quantitative real-time polymerase chain reaction and Western blot, we found that siRNA/APP decreases MMP-2 expression in vitro. Our study provides a novel clue that APP is involved in the extramedullary infiltration of leukemia by MMP-2.

Keywords

APP EMI AML/ETO+ MMP-2 Kasumi-1 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  1. 1.Department of Hematology, Nanfang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Institute of Genetic EngineeringSouthern Medical UniversityGuangzhouChina

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