Tumor Biology

, Volume 33, Issue 5, pp 1739–1744 | Cite as

Opposite roles of LPA1 and LPA3 on cell motile and invasive activities of pancreatic cancer cells

  • Kohei Kato
  • Kyohei Yoshikawa
  • Eriko Tanabe
  • Misaho Kitayoshi
  • Rie Fukui
  • Nobuyuki Fukushima
  • Toshifumi TsujiuchiEmail author
Research Article


Lysophosphatidic acid (LPA) interacts with at least six G protein-coupled transmembrane LPA receptors. Recently, it has been demonstrated that each LPA receptor acts as a positive or negative regulator of cellular function. In the present study, to assess a biological role of LPA receptors on cell migration of pancreatic cancer cells, we generated LPA receptor-1 (LPA1) and LPA3 knockdown cells from hamster pancreatic cancer cells by transfection with short hairpin RNA plasmids and measured their cell motile and invasive abilities. In cell motility and invasion assay, a Cell Culture Insert, coated with or without a Matrigel, was used. While the cell motility and invasion of Lpar1 knockdown cells were markedly enhanced than those of control cells, Lpar3 knockdown cells showed significantly lower cell motility and invasion. Moreover, to investigate an involvement of LPA1 and LPA3 in the development of pancreatic cancers, we also measured the expression levels of Lpar1 and Lpar3 genes in hamster pancreatic duct adenocarcinomas (PDAs) induced by a nitroso compound. The expressions of Lpar1 gene in PDAs were significantly lower than those in normal pancreatic tissues. By contrast, the elevated expressions of Lpar3 gene were detected in PDAs. We thus demonstrate that LPA1 and LPA3 play the different roles on cell migration ability of pancreatic cancer cells, suggesting the opposite effects via LPA1 and LPA3 may contribute to the pathogenesis of pancreatic cancers in hamsters.


LPA LPA receptor Cell migration Invasion Pancreatic cancer 



This study was supported in part by a grant-in-aid (20591765) for Scientific Research from Ministry of Education, Culture, Sports, Science and Technology of Japan, and by grants (21321201) from the Ministry of Health, Labor and Welfare of Japan, and by grants (RK-027) from the Faculty of Science and Engineering, Kinki University.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Kohei Kato
    • 1
  • Kyohei Yoshikawa
    • 1
  • Eriko Tanabe
    • 1
  • Misaho Kitayoshi
    • 1
  • Rie Fukui
    • 1
  • Nobuyuki Fukushima
    • 2
  • Toshifumi Tsujiuchi
    • 1
    Email author
  1. 1.Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and EngineeringKinki UniversityHigashiosakaJapan
  2. 2.Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and EngineeringKinki UniversityHigashiosakaJapan

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