Tumor Biology

, Volume 33, Issue 5, pp 1455–1465 | Cite as

MicroRNA-10b promotes migration and invasion through CADM1 in human hepatocellular carcinoma cells

  • Qing-jun Li
  • Liang Zhou
  • Fan Yang
  • Guo-xia Wang
  • Hang Zheng
  • De-sheng Wang
  • Yong He
  • Ke-feng Dou
Research Article


MicroRNA-10b (miR-10b) was recently reported to be dysregulated in some types of cancer and to play a role in invasion and metastasis. However, effects and potential mechanisms of action of miR-10b in the metastasis of hepatocellular carcinoma (HCC) have not been explored. In this study, we confirmed that miR-10b is highly expressed in metastatic HCC tissues and in metastatic HCC cell lines by qRT-PCR. Moreover, patients with higher miR-10b expression had significantly poorer overall survival, and high miR-10b expression was an independent predictor of poor prognosis. Inhibition of miR-10b reduced cell migration and invasion in MHCC97H cells, whereas over-expression of miR-10b in HepG2 cells increased cell migration and invasion. Bioinformatics and luciferase reporter assays revealed that miR-10b binds the 3′-UTR of CADM1 mRNA and represses its translation. Western blot and qRT-PCR showed that CADM1 is inhibited by miR-10b over-expression. Silencing of CADM1 resulted in substantially increased cell motility and invasion similar to that observed with over-expression of miR-10b in HepG2 cells. These results suggest that miR-10b may positively regulate the invasion and metastasis of HCC through targeting CADM1.


Hepatocellular carcinoma miR-10b CADM1 Migration Invasion 



This work was supported by grants from the Major Program of the National Natural Science Foundation of China [grant no. 81030010/H0318].

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Qing-jun Li
    • 1
    • 2
  • Liang Zhou
    • 1
  • Fan Yang
    • 1
  • Guo-xia Wang
    • 3
  • Hang Zheng
    • 2
  • De-sheng Wang
    • 1
  • Yong He
    • 1
  • Ke-feng Dou
    • 1
  1. 1.Department of Hepatobiliary Surgery, Xijing Hospitalthe Fourth Military Medical UniversityXi’anChina
  2. 2.Department of General Surgery, the Third Affiliated HospitalXinxiang Medical UniversityXinxiangChina
  3. 3.Department of Biochemistry and Molecular Biologythe Fourth Military Medical UniversityXi’anChina

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