Tumor Biology

, Volume 33, Issue 2, pp 561–569

Detection of circulating tumor cells and tumor stem cells in patients with breast cancer by using flow cytometry

A valuable tool for diagnosis and prognosis evaluation
Research Article

Abstract

Circulating tumor stem cells (CTSC), a subpopulation of circulating tumor cells (CTC), may lead to recurrent diseases. The aim of this study was to detect CTC (CD45EpCAM+) and CTSC (CD45EpCAM+CD44+CD24) of breast cancer (BC) patients, as well as to explore their clinical relevance. CTC and CTSC in peripheral blood (PB) of 45 female BC patients were detected by using flow cytometry (FCM). SKBR-3 cells were mixed with MNC of four healthy volunteers at different ratios in order to evaluate the sensitivity of FCM. Real-time quantitative polymerase chain reaction (QRT-PCR) was conducted and compared with FCM. The expression of EPCAM between CTC < 50 and CTC ≥ 50 groups (19.98 ± 23.93 versus 29.46 ± 29.27 × 10−5), and the expression of CD44 between CTSC negative and positive groups (0.85 ± 0.91 versus 0.81 ± 0.75) were statistically the same. FCM had higher specificity than QRT-PCR. Statistical differences were obtained between CTC < 50 and CTC ≥ 50 groups among different TNM stages, histology stages, estrogen receptor (ER) status and progesterone receptor (PR) status (P < 0.05). Statistical differences between CTSC negative and positive groups within different TNM stages and regional lymph node metastasis (RLNM) status (P < 0.05) were also obtained. Moreover, the percentage of CTC on CD45 negative cells (CD45C) among different clinical pathology was statistically different, P = 0.000. Additionally, the percentage of CTSC on CD45C with TNM stage was rising (0: 0.00 ± 0.00‰, I: 0.03 ± 0.05‰, II: 0.06 ± 0.14‰, III: 0.10 ± 0.09‰, IV: 0.29 ± 0.35‰, P = 0.034). Statistical difference in the percentage of CTSC on CD45C among different RLNM status (P = 0.001) was also obtained. FCM to detect CTC and CTSC may be used to diagnose disease at early stage, to guide clinical therapy or to predict prognosis.

Keywords

Breast cancer Circulating tumor cells Circulating tumor stem cells Flow cytometry 

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2012

Authors and Affiliations

  • Ningfang Wang
    • 1
  • Lan Shi
    • 2
  • Huiyu Li
    • 1
  • Yanjie Hu
    • 1
  • Wen Du
    • 1
  • Wei Liu
    • 1
  • Jin’e Zheng
    • 1
  • Shiang Huang
    • 1
  • Xincai Qu
    • 2
  1. 1.Department of Center for Stem Cell Research and Application, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Thyroid and Mammary Gland, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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