Tumor Biology

, Volume 32, Issue 3, pp 501–508

Differential expression pattern of estrogen receptors, aromatase, and sulfotransferase in breast cancer tissue and corresponding lymph node metastases

  • Daphne Gschwantler-Kaulich
  • Anneliese Fink-Retter
  • Klaus Czerwenka
  • Gernot Hudelist
  • Axel Kaulich
  • Ernst Kubista
  • Christian F. Singer
Research Article

Abstract

Patients with hormone receptor positive breast cancer who are treated with endocrine therapy generally have a good prognosis. However, resistance to hormonal therapy and progression occurs, and the reasons for this are manifold. It has been proposed that the local estrogenic environment has a role in the process of local invasion and progression. We have determined the expression pattern of estrogen receptor α, estrogen receptor β, and the epithelial and stromal expression of the estrogen-metabolizing enzymes aromatase and sulfotransferase by immunohistochemistry in tissue arrays, containing 50 paraffin-embedded sets of tissues obtained from breast cancer and from corresponding metastatic axillary lymph nodes of the same patients. We have found statistically significant higher estrogen receptors α and β expression in primary tumors than in corresponding lymph node metastases (p = 0.0004 and p = 0.003, respectively). Aromatase was also expressed more frequently in epithelial as well as in stromal cells of the malignant tumor when compared to according lymph node metastases (p = 0.08 and p = 0.12, respectively). While in lymph node metastases only estrogen receptor α and stromal aromatase expression were correlated (p = 0.01), significant associations were seen between the estrogen receptor β and stromal aromatase, and epithelial sulfotransferase (p = 0.0006 and p = 0.03, respectively) in the primary tumor. We hypothesize that the decreased expression of local estrogens by aromatase, in combination with a decreased expression of estrogen receptors α and β in lymphatic metastases, renders these metastases hormone insensitive and could contribute to the poor response to endocrine therapy that is often seen in nodal-positive tumors.

Keywords

Aromatase Estrogen sulfotransferase Estrogen receptor Breast cancer Lymph node metastases 

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2010

Authors and Affiliations

  • Daphne Gschwantler-Kaulich
    • 1
  • Anneliese Fink-Retter
    • 1
  • Klaus Czerwenka
    • 2
  • Gernot Hudelist
    • 1
    • 4
  • Axel Kaulich
    • 1
  • Ernst Kubista
    • 1
    • 3
  • Christian F. Singer
    • 5
  1. 1.Division of Special GynaecologyUniversity of Vienna Medical CenterViennaAustria
  2. 2.Division of GynaecopathologyMedical University of ViennaViennaAustria
  3. 3.Ludwig-Boltzmann-Institute of Clinical Experimental OncologyMedical University of ViennaViennaAustria
  4. 4.Department of Obstetrics and GynaecologyVillach Medical CentreVillachAustria
  5. 5.Department of OB/GYNMedical University of ViennaViennaAustria

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