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Tumor Biology

, Volume 31, Issue 3, pp 199–207 | Cite as

Calcineurin promotes proliferation, migration, and invasion of small cell lung cancer

  • Yan Liu
  • Ye Zhang
  • Jie Min
  • Li-Li Liu
  • Ning-Qiang Ma
  • Ying-Ming Feng
  • Dong Liu
  • Ping-zhong Wang
  • De-Dong Huang
  • Yan Zhuang
  • He-Long Zhang
Research Article

Abstract

A novel role for calcineurin (Cn) has been reported recently regarding the oncogenic potential in pancreatic and colorectal cancer. The aim of this study was to investigate the putative causal role calcineurin could play in the development of lung cancer with bone metastases. We found that CnAα, an isoform of calcineurin, was significantly overexpressed in lung cancer tissues with bone metastasis as compared to tumors with non-bone metastases as investigated by RT-PCR. Strong nuclear staining of tumor cells was observed in small cell lung cancer tissues with bone metastasis. Conversely, cytoplasmic staining of tumor cells was observed in small cell lung cancer tissues with non-bone metastasis. Western blots of nuclear proteins from lung cancer tissues indicated that CnAα was highly expressed in lung cancer tissues with bone metastases, but not in those with non-bone metastases. In vitro, it was demonstrated that the CnAα gene obviously promoted cell proliferation and inhibited cell apotosis. The CnAα gene affected the cell cycle and promoted G1➝S transition in SBC-3 cells. Transfection with the CnAα gene promoted cell migration and invasion. These results indicated that CnAα may affect the biological behavior of the human small cell lung cancer cell line SBC-3 in vitro and may be a candidate tumor promotor gene for developing bone metastases.

Keywords

Calcineurin Small cell lung cancer Bone metastasis Proliferation Migration Invasion 

Notes

Acknowledgments

This study was supported by National Natural Science Foundation of China (No. 30873028)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2010

Authors and Affiliations

  • Yan Liu
    • 1
  • Ye Zhang
    • 2
  • Jie Min
    • 1
  • Li-Li Liu
    • 1
  • Ning-Qiang Ma
    • 1
  • Ying-Ming Feng
    • 1
  • Dong Liu
    • 1
  • Ping-zhong Wang
    • 2
  • De-Dong Huang
    • 2
  • Yan Zhuang
    • 2
  • He-Long Zhang
    • 1
  1. 1.Department of Oncology, Tangdu HospitalFourth Military Medical UniversityXi’anChina
  2. 2.Center of Diagnosis and Treatment for Infectious Diseases, Tangdu HospitalThe Fourth Military Medical UniversityXi’anChina

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