Tumor Biology

, Volume 31, Issue 2, pp 103–112

Antibody array-based technologies for cancer protein profiling and functional proteomic analyses using serum and tissue specimens

Research Article

Abstract

In the context of other proteomic technologies, targeted antibody arrays are strongly contributing for protein profiling and functional proteomics analyses in serum specimens. Protein-protein interactions, post-translational modifications, and interaction between protein and DNA or RNA can all shift the activity of a protein from what would have been predicted by its level of transcription. Functional proteomics studies the interaction of proteins within their cellular environment to determine how a given protein accomplishes its specific cellular task. Accordingly, the promise of protein profiling and functional proteomics is that by chronicling the function of aberrant or over-expressed proteins, it will be possible to characterize the mechanism of the disease-sustaining proteins. The further understanding of the disease networks will eventually lead to targeted cancer therapy and specific biomarkers for diagnosis, prognosis or therapeutic response prediction based on disease specific proteins. This review describes how such strategies reported to date in serum specimens may assist in characterizing tumor biology, and for the diagnosis, surveillance, prognosis, and potentially for predictive and therapeutic purposes for patients affected with solid and hematological neoplasias.

Keywords

Antibody arrays Functional proteomics Serum 

Abbreviations

DNA

desoxirribonucleic acid

RNA

ribonucleic acid

MS

mass spectrometry

2D

two-dimensional electrophoresis

TAA

tumor-associated antigen

Cy3

cyanine 3

Cy5

cyanine 5

IL

interleukin

PSA

prostate-specific antigen

SA

streptavidin

SPRI

surface plasmon resonance imaging

VEGF

vascular endothelial growth factor

PrEST

protein epitope signature tags

OCT

optimal cutting temperature

RCA

rolling circle amplification

RLS

Resonance light Scattering

ECL

Enhanced ChemiLuminescence

TSA

tyramide signal amplification

TIF

tumor interstitial fluid

CSF

cerebrospinal fluid

HUPO

Human Proteome Organization

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2010

Authors and Affiliations

  1. 1.Tumor Markers Group, Spanish National Cancer Research CenterMadridSpain

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