Toxicoproteomic analysis of deltamethrin exposure in neuroblastoma cell lines
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Deltamethrin, a type II pyrethroid insecticide, has been widely used due to its high efficacy against insects and low toxicity to mammals. However, recently, potential adverse health effects during deltamethrin exposure have been reported.
A proteomic analysis using two-dimensional electrophoresis with mass spectrometry was performed, and the proteomic data were validated by western blotting. Antioxidant enzyme activity was also measured by spectrophotometric analysis.
The 13 differentially expressed proteins, of which 8 were up-regulated and 5 down-regulated, are involved in a variety of cellular functions including protein folding, cytoskeleton regulation, and splicing process. The activities of antioxidant enzymes and acetylcholinesterase were also altered by deltamethrin exposure.
Differential protein expression caused by deltamethrin exposure in neuroblastoma cell lines could allow us to develop biomarkers for deltamethrin toxicity, and pursue future studies to assess the molecular mechanisms of deltamethrin toxicity.
KeywordsDeltamethrin Neuroblastoma cells Proteomics
This study was supported by Soonchunhyang University.
MJK performed the experiments and wrote the initial draft of manuscript. MYL designed the research and wrote the final version of manuscript.
Compliance with ethical standards
Conflict of interest
Min-Joo Kang and Mi-Young Lee declare that they have no conflict of interest.
Human and animal rights
The article does not contain any studies with humans or animals and this study was performed following institutional and national guidelines.
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