Molecular & Cellular Toxicology

, Volume 16, Issue 1, pp 93–101 | Cite as

Toxicoproteomic analysis of deltamethrin exposure in neuroblastoma cell lines

  • Min-Joo Kang
  • Mi-Young LeeEmail author
Original Article



Deltamethrin, a type II pyrethroid insecticide, has been widely used due to its high efficacy against insects and low toxicity to mammals. However, recently, potential adverse health effects during deltamethrin exposure have been reported.


A proteomic analysis using two-dimensional electrophoresis with mass spectrometry was performed, and the proteomic data were validated by western blotting. Antioxidant enzyme activity was also measured by spectrophotometric analysis.


The 13 differentially expressed proteins, of which 8 were up-regulated and 5 down-regulated, are involved in a variety of cellular functions including protein folding, cytoskeleton regulation, and splicing process. The activities of antioxidant enzymes and acetylcholinesterase were also altered by deltamethrin exposure.


Differential protein expression caused by deltamethrin exposure in neuroblastoma cell lines could allow us to develop biomarkers for deltamethrin toxicity, and pursue future studies to assess the molecular mechanisms of deltamethrin toxicity.


Deltamethrin Neuroblastoma cells Proteomics 



This study was supported by Soonchunhyang University.

Author contributions

MJK performed the experiments and wrote the initial draft of manuscript. MYL designed the research and wrote the final version of manuscript.

Compliance with ethical standards

Conflict of interest

Min-Joo Kang and Mi-Young Lee declare that they have no conflict of interest.

Human and animal rights

The article does not contain any studies with humans or animals and this study was performed following institutional and national guidelines.


  1. Baggerman G, Vierstraete E, De Loof A, Schoofs L (2005) Gel-based versus gel-free proteomics: a review. Comb Chem High Throughput Screen 8:669–677PubMedGoogle Scholar
  2. Bai B et al (2019) Peroxiredoxin2 downregulation enhances hepatocellular carcinoma proliferation and migration, and is associated with unfavorable prognosis in patients. Oncol Rep 41:1539–1548PubMedPubMedCentralGoogle Scholar
  3. Caballero JP et al (2019) Nanoencapsulated deltamethrin as synergistic agent potentiates insecticide effect of indoxacarb through an unusual neuronal calcium-dependent mechanism. Pestic Biochem Physiol 157:1–12Google Scholar
  4. Cabello G et al (2001) A rat mammary tumor model induced by the organophosphorous pesticides parathion and malathion, possibly through acetylcholinesterase inhibition. Environ Health Perspect 109:471–479PubMedPubMedCentralGoogle Scholar
  5. Cao W et al (2018) Determination of deltamethrin and its toxicity biomarkers in rabbit urine by high performance liquid chromatography-tandem mass spectrometry. Se Pu 36:523–530PubMedGoogle Scholar
  6. Chauvin S, Sobel A (2015) Neuronal stathmins: a family of phosphoproteins cooperating for neuronal development, plasticity and regeneration. Prog Neurobiol 126:1–18PubMedGoogle Scholar
  7. Chen M et al (2019) Molecular evidence of sequential evolution of DDT-and pyrethroid-resistant sodium channel in aedes aegypti. PLoS Negl Trop Dis 13:e0007432PubMedPubMedCentralGoogle Scholar
  8. Chrustek A et al (2018) Current research on the safety of pyrethroids used as insecticides. Medicina (Kaunas) 54:61Google Scholar
  9. Cook JA, Mitchell JB (1989) Viability measurements in mammalian cell systems. Anal Biochem 179:1–7PubMedGoogle Scholar
  10. Danh HC, Benedetti MS, Dostert P (1983) Differential changes in superoxide dismutase activity in brain and liver of old rats and mice. J Neurochem 40:1003–1007PubMedGoogle Scholar
  11. Ding R et al (2017) The implication of p66shc in oxidative stress induced by deltamethrin. Chem Biol Interact 278:162–169PubMedGoogle Scholar
  12. Dinis-Oliveira RJ et al (2006) Acute paraquat poisoning: report of a survival case following intake of a potential lethal dose. Pediatr Emerg Care 22:537–540PubMedGoogle Scholar
  13. Doi H et al (2006) Motor neuron disorder simulating ALS induced by chronic inhalation of pyrethroid insecticides. Neurology 67:1894–1895PubMedGoogle Scholar
  14. El Golli-Bennour E et al (2019) Protective effects of kegir against deltamethrin-induced hepatotoxicity in rats. Environ Sci Pollut Res Int 26:18856–18865PubMedGoogle Scholar
  15. Ellman GL, Courtney KD, Andres V Jr, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95PubMedGoogle Scholar
  16. Ensibi C et al (2013) Effects of deltamethrin on biometric parameters and liver biomarkers in common carp (Cyprinus carpio L.). Environ Toxicol Pharmacol 36:384–391PubMedGoogle Scholar
  17. Flohé L, Günzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105:114–120PubMedGoogle Scholar
  18. Fulton MH, Key PB (2001) Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environ Toxicol Chem 20:37–45PubMedGoogle Scholar
  19. Gautrey H et al (2015) SRSF3 and hnRNP H1 regulate a splicing hotspot of HER2 in breast cancer cells. RNA Biol 12:1139–1151PubMedPubMedCentralGoogle Scholar
  20. Glorieux C, Calderon PB (2017) Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem 398:1095–1108PubMedGoogle Scholar
  21. Hadwan MH (2018) Simple spectrophotometric assay for measuring catalase activity in biological tissues. BMC Biochem 19:1–8Google Scholar
  22. Hileman EA, Achanta G, Huang P (2001) Superoxide dismutase: an emerging target for cancer therapeutics. Expert Opin Ther Targets 5:697–710PubMedGoogle Scholar
  23. Ingkaninan K, Temkitthawon P, Chuenchom K, Yuyaem T, Thongnoi W (2003) Screening for acetylcholinesterase inhibitory activity in plants used in thai traditional rejuvenating and neurotonic remedies. J Ethnopharmacol 89:261–264PubMedGoogle Scholar
  24. Jia ZZ, Zhang JW, Zhou D, Xu DQ, Feng XZ (2019) Deltamethrin exposure induces oxidative stress and affects meiotic maturation in mouse oocyte. Chemosphere 223:704–713PubMedGoogle Scholar
  25. Kumar A, Sharma R, Rana D, Sharma N (2019a) Protective effect of alpha-tocopherol in deltamethrin induced immunotoxicity. Endocr Metab Immune Disord Drug Targets 19:171–184PubMedGoogle Scholar
  26. Kumar A, Gupta M, Sharma R, Sharma N (2019b) Deltamethrin induced immunotoxicity and its protection by quercetin: an experimental study. Endocr Metab Immune Disord Drug Targets 19:1Google Scholar
  27. Lu W et al (2014) Peroxiredoxin 2 is upregulated in colorectal cancer and contributes to colorectal cancer cells’ survival by protecting cells from oxidative stress. Mol Cell Biochem 387:261–270PubMedGoogle Scholar
  28. Lu Q et al (2018) Deltamethrin toxicity: a review of oxidative stress and metabolism. Environ Res 170:260–281PubMedGoogle Scholar
  29. Mannervik B (1999) Measurement of glutathione reductase activity. Curr Protoc Toxicol 7:1–4Google Scholar
  30. Min CW et al (2017) Gel-based and gel-free proteome data associated with controlled deterioration treatment of glycine max seeds. Data Br 15:449–453Google Scholar
  31. Nakamura M et al (2006) Phosphoproteomic profiling of human SH-SY5Y neuroblastoma cells during response to 6-hydroxydopamine-induced oxidative stress. Biochim Biophys Acta 1763:977–989PubMedGoogle Scholar
  32. Oda SS, El-Maddawy ZK (2012) Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats. Exp Toxicol Pathol 64:813–819PubMedGoogle Scholar
  33. Pitzer EM et al (2019) Deltamethrin exposure daily from postnatal day 3–20 in sprague-dawley rats causes long-term cognitive and behavioral deficits. Toxicol Sci 169:511–523PubMedGoogle Scholar
  34. Ríos JC et al (2003) Tribromophenol induces the differentiation of SH-SY5Y human neuroblastoma cells in vitro. Toxicol In Vitro 17:635–641PubMedGoogle Scholar
  35. Rubin CI, Atweh GF (2004) The role of stathmin in the regulation of the cell cycle. J Cell Biochem 93:242–250PubMedGoogle Scholar
  36. Ryu AR, Kim YW, Lee MY (2019) Chlorin e6 and halogen light as a sebostatic photomedicine modulates linoleic acid-induced lipogenesis. Mol Cell Toxicol 15:49–56Google Scholar
  37. Shim DH, Lim JW, Kim H (2015) Differentially expressed proteins in nitric oxide-stimulated NIH/3T3 fibroblasts: implications for inhibiting cancer development. Yonsei Med J 56:563–571PubMedPubMedCentralGoogle Scholar
  38. Shishkin SS, Kovalev LI, Pashintseva NV, Kovaleva MA, Lisitskaya K (2019) Heterogeneous nuclear ribonucleoproteins involved in the functioning of telomeres in malignant cells. Int J Mol Sci 20:745PubMedCentralGoogle Scholar
  39. Shrivastava R, Köster D, Kalme S, Mayor S, Neerathilingam M (2015) Tailor-made ezrin actin binding domain to probe its interaction with actin in-vitro. PLoS One 10:1–12Google Scholar
  40. Skandrani D et al (2003) Effect of selected insecticides on growth rate and stress protein expression in cultured human A549 and SH-SY5Y cells. Toxicol In Vitro 20:1378–1386Google Scholar
  41. Souza MF et al (2018) Deltamethrin intranasal administration induces memory, emotional and tyrosine hydroxylase immunoreactivity alterations in rats. Brain Res Bull 142:297–303PubMedGoogle Scholar
  42. Suenaga S et al (2013) Human pancreatic cancer cells with acquired gemcitabine resistance exhibit significant up-regulation of peroxiredoxin-2 compared to sensitive parental cells. Anticancer Res 33:4821–4826PubMedGoogle Scholar
  43. Traverso N et al (2013) Role of glutathione in cancer progression and chemoresistance. Oxidative Med Cell Longev 2013:1–10Google Scholar

Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Medical ScienceSoonchunhyang UniversityAsanKorea
  2. 2.Department of Medical BiotechnologySoonchunhyang UniversityAsanKorea

Personalised recommendations