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Molecular & Cellular Toxicology

, Volume 16, Issue 1, pp 93–101 | Cite as

Toxicoproteomic analysis of deltamethrin exposure in neuroblastoma cell lines

  • Min-Joo Kang
  • Mi-Young LeeEmail author
Original Article
  • 30 Downloads

Abstract

Background

Deltamethrin, a type II pyrethroid insecticide, has been widely used due to its high efficacy against insects and low toxicity to mammals. However, recently, potential adverse health effects during deltamethrin exposure have been reported.

Methods

A proteomic analysis using two-dimensional electrophoresis with mass spectrometry was performed, and the proteomic data were validated by western blotting. Antioxidant enzyme activity was also measured by spectrophotometric analysis.

Results

The 13 differentially expressed proteins, of which 8 were up-regulated and 5 down-regulated, are involved in a variety of cellular functions including protein folding, cytoskeleton regulation, and splicing process. The activities of antioxidant enzymes and acetylcholinesterase were also altered by deltamethrin exposure.

Conclusion

Differential protein expression caused by deltamethrin exposure in neuroblastoma cell lines could allow us to develop biomarkers for deltamethrin toxicity, and pursue future studies to assess the molecular mechanisms of deltamethrin toxicity.

Keywords

Deltamethrin Neuroblastoma cells Proteomics 

Notes

Acknowledgements

This study was supported by Soonchunhyang University.

Author contributions

MJK performed the experiments and wrote the initial draft of manuscript. MYL designed the research and wrote the final version of manuscript.

Compliance with ethical standards

Conflict of interest

Min-Joo Kang and Mi-Young Lee declare that they have no conflict of interest.

Human and animal rights

The article does not contain any studies with humans or animals and this study was performed following institutional and national guidelines.

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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Medical ScienceSoonchunhyang UniversityAsanKorea
  2. 2.Department of Medical BiotechnologySoonchunhyang UniversityAsanKorea

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