Molecular & Cellular Toxicology

, Volume 12, Issue 3, pp 265–271 | Cite as

Amygdalin inhibits HSC-T6 cell proliferation and fibrosis through the regulation of TGF-β/CTGF

  • Huanhuan Luo
  • Liang Li
  • Jianbang Tang
  • Fengxue Zhang
  • Fang Zhao
  • Da Sun
  • Fengling Zheng
  • Xinhua WangEmail author
Original Paper


A mygdalin is one of the nitrilosides that was widely used to treat cancer, inhibit liver fibrosis. In the present study, the aim was to determine the antifibrotic potential of amygdalin and examine its mechanisms of action in vitro. Firstly, we found amygdalin significantly inhibited HSC-T6 cells proliferation. Both mRNA and protein of transforming growth factor-β (TGF-β) were decreased in HSC-T6 cells during amygdalin treatment. Secondly, to investigate functional role of TGF-β, both TGF-β over-expression vector and siRNA against TGF-β were transfected into HSC-T6 cells respectively. The results showed that over-expression of TGF-β promoted proliferation of HSC-T6 cells, whereas TGF-β knockdown inhibited cell viability. Moreover, our data even indicated that TGF-β could promote cell proliferation independent of amygdalin treatment. Finally, we found amygdalin could inhibit expression of the classical fibrotic factor αSMA, which suggested an antifibrotic effect of amygdalin. While the TGF-β antagonized anti-fibrotic effect of amygdalin. To assess the mechanisms, we examined expression of CTGF in cultured HSC-T6 cells. Our results showed that CTGF was down-regulated in HSCT6 cell treated by amygdalin, but was up-regulated when exogenous TGF-β introduced. As CTGF was one of the downstream factors in the TGF-β pathway. These might suggest that amygdalin inhibited HSC-T6 cells proliferation and fibrosis via TGF-β/CTGF pathway.


Amygdalin HSC-T6 cells Fibrosis TGF-β/CTGF 


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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Huanhuan Luo
    • 1
  • Liang Li
    • 2
  • Jianbang Tang
    • 3
  • Fengxue Zhang
    • 1
  • Fang Zhao
    • 1
  • Da Sun
    • 4
  • Fengling Zheng
    • 1
  • Xinhua Wang
    • 5
    Email author
  1. 1.Tropical Medicine InstituteGuangzhou university of Chinese medicineGuangzhouChina
  2. 2.General DepartmentHospital of Traditional Chinese Medicine of ZhongshanZhongshanChina
  3. 3.Bone Disease Research InstituteHospital of Traditional Chinese Medicine of ZhongshanZhongshanChina
  4. 4.BGI-ShenzhenShenzhenChina
  5. 5.Headmaster’s officeGuangzhou Medical UniversityGuangzhouChina

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