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Protective effect of a novel herbmedicine, Hepad, on apoptosis of SH-SY5Y cells and a rat model of Parkinson’s disease

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Abstract

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by loss of dopaminergic neurons in the substantia nigra pars compacta. In this study, we investigated the effects of a novel herb formula, Hepad, on PD. Dose-dependent treatment with 1-methyl-4-phenylpyridinium (MPP+) decreased the viability of SH-SY5Y cells, and Hepad inhibited the toxic effect of MPP+. Hepad blocked the production of reactive oxygen species (ROS) induced by MPP+ in SH-SY5Y cells, and suppressed the activation of caspase 9 and caspase 3 due to MPP+. A rat model of PD was generated by 6-hydroxydopamine (6-OHDA) injection into the left medial forebrain bundle (MFB) of SD rats. In D-amphetamine sulfate-induced rotational behavioral tests, Hepad administration attenuated circling behavior relative to the 6-OHDA-treated disease group. In addition, Hepad treatment significantly increased the tyrosine hydroxylase (TH)-positive cells in the substantia nigra pars compacta (SNpc) that had decreased in response to 6-OHDA treatment (P<0.05). OX-6 expression, which indicates the presence of microglial cells, decreased significantly after treatment of Hepad in contrast to the 6-OHDA-treated disease group (P<0.05). These results indicate that Hepad may be a useful neuroprotective material for the treatment of neurodegenerative disorders such as PD.

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Authors and Affiliations

  1. Department of Senior Healthcare, BK21 plus program, Graduate School, Eulji University, Daejeon, 301-746, Korea

    Seung Yeop Baek, Da Hye Kim, Ayoung Gu, Seong Yeol Kim & In Sik Kim

  2. Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon, 301-746, Korea

    Na Rae Lee & In Sik Kim

  3. Department of Anatomy and Neuroscience, School of Medicine, Eulji University, Daejeon, 301-768, Korea

    Dae-Yong Song

  4. Department of Pathology, College of Oriental Medicine, Daejeon University, Daejeon, 300-716, Korea

    Dong-Hee Kim, Hak Joo Choi & Byung-Jun Park

  5. Young Jin Korea Medical Clinic, Sun Cheon, 540-320, Korea

    Byung-Jun Park

Authors
  1. Seung Yeop Baek
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  2. Na Rae Lee
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  3. Da Hye Kim
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  4. Ayoung Gu
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  5. Seong Yeol Kim
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  7. Dong-Hee Kim
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  9. Byung-Jun Park
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  10. In Sik Kim
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Correspondence to Byung-Jun Park or In Sik Kim.

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Baek, S.Y., Lee, N.R., Kim, D.H. et al. Protective effect of a novel herbmedicine, Hepad, on apoptosis of SH-SY5Y cells and a rat model of Parkinson’s disease. Mol. Cell. Toxicol. 11, 223–230 (2015). https://doi.org/10.1007/s13273-015-0021-7

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  • DOI: https://doi.org/10.1007/s13273-015-0021-7

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