Molecular & Cellular Toxicology

, Volume 10, Issue 3, pp 293–301 | Cite as

The effect of metal-doped TiO2 nanoparticles on zebrafish embryogenesis

  • Hyung-Geun Park
  • Jung In Kim
  • Misook Kang
  • Min-Kyeong Yeo
Original Paper
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Abstract

In this study, we manufactured metal-doped TiO2 nanoparticles using several transition metals (Mn, Fe, Ni, and Cu). Their physicochemical properties were evaluated by transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, and UV-visible spectra. The energy efficiency of the metals increased in the order of Ni-<Cu-<Mn-<Fe-TiO2. For toxicity testing, zebrafish embryos were exposed to 4 mg L−1 of metal-doped TiO2 nanoparticles. We determined the survival rate (%) and abnormal morphology rate (%) of the exposed embryos and also observed apoptosis and necrosis using confocal microscopy. The metal TiO2 nanoparticles showed acute toxicity in the order of Mn-<Cu-<Ni-≤Fe-TiO2. Although the Fe-TiO2 NPs group demonstrated the highest photocatalytic performance, it also exhibited the highest toxic effects. Among the metals, the Mn-TiO2 NPs group demonstrated improved photocatalysis compared to the other samples except for the Fe-TiO2 NPs along with the lowest toxic effects. For these reasons, the most suitable doping metal was the Mn-TiO2 NPs group considering its energy activity and environmental impacts.

Keywords

Metal-doped TiO2 nanoparticles Photocatalysis Energy efficiency Toxicity Zebrafish embryos 
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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Hyung-Geun Park
    • 1
  • Jung In Kim
    • 1
  • Misook Kang
    • 2
  • Min-Kyeong Yeo
    • 1
  1. 1.Department of Environmental Science and Environmental Research Center, College of EngineeringKyung Hee UniversityGyeonggi-doKorea
  2. 2.Department of Chemistry, College of ScienceYeungnam UniversityGyeongsan, GyeongbukKorea

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