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Molecular & Cellular Toxicology

, Volume 9, Issue 1, pp 29–36 | Cite as

Cytotoxicity of recombinant immunotoxin containing lectin A chain from Korean mistletoe

  • Joonseok Cho
  • Inbo Kim
  • Ju-Seong Jeong
  • Seung-Pil Jung
  • Tae-Bong Kang
  • Jong-Bae Kim
Original Paper
  • 283 Downloads

Abstract

Toxin moiety in immunotoxin (IT) plays an important role in cytotoxicity of target cells. We constructed a recombinant immunotoxin (RIT), which is composed of a recombinant lectin A chain from Korean mistletoe (AKM) and a single-chain Fv (scFv) of an anti-carcinoembryonic antigen (CEA) monoclonal antibody. This single-chain IT was expressed in E. coli, purified, and refolded as a soluble protein. The resulting recombinant protein displayed strong cytotoxicity against CEA-expressing cancer cells but only minimal cytotoxicity against non CEA-expressing cells. The present study, we suggest that AKM may be a potential toxin candidate for development of an IT.

Keywords

Carcinoembryonic antigen Korean mistletoe Lectin A chain Cytotoxicity Immunotoxin 

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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Joonseok Cho
    • 1
  • Inbo Kim
    • 1
  • Ju-Seong Jeong
    • 1
  • Seung-Pil Jung
    • 2
  • Tae-Bong Kang
    • 3
  • Jong-Bae Kim
    • 1
  1. 1.School of Life ScienceHandong UniversityPohang, GyeongbukKorea
  2. 2.Department of Family MedicineYeungnam University College of MedicineDaeguKorea
  3. 3.Department of Biotechnology, College of Biomedical and Health ScienceKonkuk UniversityChung-JuKorea

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