Molecular & Cellular Toxicology

, 7:271

Expression profiling of liver in Java medaka fish exposed to 17β-estradiol

  • Seonock Woo
  • Hye-Young Jeon
  • Taek-Kyun Lee
  • Seong-Ryul Kim
  • Seung-hoon Lee
  • Seungshic Yum
Original Paper


Java medaka (Oryzias javanicus) cDNA array was constructed and the microarray platform was used to compare the hepatic expression profiles of Java medaka fish exposed to 17β-estradiol with those of unexposed controls. Data analysis demonstrated that the expression profiles were strongly affected by 17β-estradiol exposure, with 655 genes up- or downregulated after 24 h, and 633 genes after 48 h. The differentially expressed genes were analyzed to determine the effects of 17β-estradiol exposure on the liver tissue and were classified into five functional categories: information storage and processing, cellular processes and signaling, metabolism, general function prediction only, and function unknown. Genes whose expression was upregulated more than 10-fold were predominantly associated with energy production/conversion and reproduction, and 30% of the genes whose expression was downregulated more than 10-fold were associated with carbohydrate transport and metabolism. The observed differences in the expression profiles of 7 genes (encoding apolipoprotein B, cytochrome P450 1A, glucose-6-phosphate dehydrogenase, glutamate dehydrogenase 1b, vitellogenin, selenoprotein M, and transferrin) were confirmed by quantitative RT-PCR, and their transcriptional changes in the livers of Java medaka induced by exposure to 10, 100, and 1,000 μg/L 17β-estradiol were investigated. These results should allow the development of biomarkers for the identification of 17β-estradiol contamination in the environment and provide molecular biological information on the effects of endocrine-disrupting chemical exposure on marine animals.


Oryzias javanicus Microarray 17β-estradiol Gene expression Gene ontology 


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Copyright information

© The Korean Society of Toxicogenomics and Toxicoproteomics and Springer Netherlands 2011

Authors and Affiliations

  • Seonock Woo
    • 1
  • Hye-Young Jeon
    • 1
  • Taek-Kyun Lee
    • 1
  • Seong-Ryul Kim
    • 1
  • Seung-hoon Lee
    • 2
  • Seungshic Yum
    • 1
  1. 1.South Sea Environment Research DepartmentKorea Ocean Research and Development InstituteGeojeKorea
  2. 2.Department of Biological ScienceYong In UniversityYonginKorea

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