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Transcriptomic analysis provides insight into the mechanism of salinity adjustment in swimming crab Portunus trituberculatus

  • Baoquan Gao
  • Dongfang Sun
  • Jianjian Lv
  • Xianyun Ren
  • Ping LiuEmail author
  • Jian Li
Research Article
  • 25 Downloads

Abstract

Background

Low salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the swimming crab (Portunus trituberculatus) is adaptive to relatively low salinity. However, the mechanisms underlying salinity stress responses in P. trituberculatus is not very clear.

Objectives

The primary objective of this study was to describe the salinity adaptation mechanism in P. trituberculatus.

Methods

The crabs were exposed to low salinity stress, and gill tissue was sampled at 0, 12, 36, 48 and 72 h and subjected to high throughput sequencing. Subsequently, we tested the accuracy and quality of the sequencing results, and then carried out GO and KEGG bioinformatics on the differentially expressed genes (DEG).

Results

Each sample yielded more than 1.1 Gb of clean data and 23 million clean reads. The process was divided into early (0–12 h), middle (12–48 h), and late phase (48–72 h). A total of 1971 (1373 up-regulated, 598 down-regulated), 1212 (364 up-regulated, 848 down-regulated), and 555 (187 up-regulated, 368 down-regulated) DEGs with annotations were identified during the three stages, respectively. DEGs were mainly associated with lipid metabolism energy metabolism, and signal transduction from the three stages, respectively.

Conclusion

A substantial number of genes were modified by salinity stress, along with a few important salinity acclimation pathways. This work provides valuable information on the salinity adaptation mechanism in P. trituberculatus. In addition, the comprehensive transcript sequences reported in this study provide a rich resource for identification of novel genes in this and other crab species.

Keywords

Transcriptomics Salinity adjustment Swimming crab Portunus trituberculatus 

Notes

Acknowledgements

This research was supported by the National Natural Science Foundation of China (Grant nos. 41506186, 41876187 and 41576147), and the Key Research and Development Plan of Shandong Province (2016GSF115028), Jiangsu Science and Technology Department (BE2017325).

Compliance with ethical standards

Conflict of interest

Baoquan Gao, Dongfang Sun, Jianjian Lv, Xianyun Ren, Ping Liu, Jian Li declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors. The study protocol was approved by the Experimental Animal Ethics Committee, Yellow Sea Research Institute, Chinese Academy of Fishery Sciences, China.

Supplementary material

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Copyright information

© The Genetics Society of Korea 2019

Authors and Affiliations

  • Baoquan Gao
    • 1
    • 2
  • Dongfang Sun
    • 1
  • Jianjian Lv
    • 1
    • 2
  • Xianyun Ren
    • 1
    • 2
  • Ping Liu
    • 1
    • 2
    Email author
  • Jian Li
    • 1
    • 2
  1. 1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoPeople’s Republic of China
  2. 2.Laboratory for Marine Fisheries and AquacultureQingdao National Laboratory for Marine Science and TechnologyQingdaoPeople’s Republic of China

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