Transcriptome analysis for identifying possible causes of post-reproductive death of Sepia esculenta based on brain tissue
The subpeduncle lobe/olfactory lobe–optic gland axis is called the endocrine regulation center of cephalopods. However, little is known about the mechanism of the subpeduncle lobe/olfactory lobe-optic gland axis regulate the sexual maturation and post-reproductive death of Sepia esculenta Hoyle.
The primary objective of this study was to provide basic information for revealing the mechanism of the subpeduncle lobe/olfactory lobe–optic axis regulating the rapid post-reproductive death of S. esculenta.
In this paper, Illumina sequencing based transcriptome analysis was performed on the brain tissue of female S. esculenta in the three key developmental stages: growth stage (BG), spawning stage (BS), and post-reproductive death stage (BA).
A total of 66.19 Gb Illumina sequencing data were obtained. A comparative analysis of the three stages showed 2609, 3333, and 170 differentially expressed genes (DEGs) in BG-vs-BA, BG-vs-BA, and BS-vs-BA, respectively. The Gene Ontology (GO) enrichment analysis of DEGs revealed that the regulation of cyclin-dependent protein serine/threonine kinase activity, oxidative phosphorylation, and respiratory chain were significantly enriched. The significant enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway identified pathways associated with the regulation of death, such as the mammalian target of rapamycin (mTOR) signaling pathway, AMPK signaling pathway, oxidative phosphorylation, and cell cycle.
The post-reproductive death of S. esculenta was found to be a complex energy steady-state regulation network system. The mTOR acted as an energy receptor and had a key role in regulating energy homeostasis.
KeywordsDEGs Golden cuttlefish Rapid post-reproductive death Sepia esculenta Subpeduncle lobe/olfactory lobe Transcriptome
This work was supported by the National Natural Science Foundation of China (31672645), and the basic scientific research service fee of the Central Scientific Research Institute (20603022016001) and Taishan Scholar Project, Shandong Province (2015-2019).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Imamura K, Ogura T, Kishimoto A, Kaminishi M, Esumi H (2001) Cell cycle regulation via p53 phosphorylation by a 5′-AMP activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside, in a human hepatocellular carcinoma cell line. Biochem Biophys Res Commun 2:562–567CrossRefGoogle Scholar
- Künstner A, Wolf JBW, Backstrom N, Whitney O, Balakrishnan CN, Day L, Edwards SV, Janes DE, Schlinger BA, Wilson RK, Jarvis ED, Warren WC, Ellegren H (2010) Comparative genomics based on massive parallel transcriptome sequencing reveals patterns of substitution and selection across 10 bird species. Mol Ecol 19(Suppl 1):266–276CrossRefGoogle Scholar
- Laplante M, Sabatini DM (2012) mTOR Signaling. Csh Perspect Biol 4(2):1Google Scholar
- Nesis KN (1987) Cephalopods of the world. Neptune City: T. F. H. Publications 1–15Google Scholar
- Ropelle ER, Pauli JR, Fernandes MF, Rocco SA, Marin RM, Morari J, Souza KK, Dias MM, Gomes-Marcondes MC, Gontijo JA, Franchini KG, Velloso LA, Saad MJ, Carvalheira JB (2008) A central role for neuronal AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) in high-protein diet-induced weight loss. Diabetes 57(3):594–605CrossRefGoogle Scholar
- Wan LY, Su W, Li B, Lei Y, Yan LY, Kang YP, Huan DX, Chen YN, Jiang HF, Liao BS (2018) Molecular analysis of formation of drought tolerance traits in peanut. Chin J Oil Crop Sci 40(3):335–343 (Chinese with English abstract)Google Scholar
- Wang L, Zhang XM, Ding PW, Liu TY, Chen SQ (2017) Reproductive behavior and mating strategy of Sepia esculenta. Acta Ecol Sin 1000-0933(6):1871–1880 (Chinese with English abstract)Google Scholar
- Wells MJ, Wells J (1959) Hormonal control of sexual maturity in octopus. J Exp Biol 36:1–33Google Scholar
- Yin YN, Liu CL, Hu P, Zhang JY, Liu SF, Zhuang ZM, Xue TM (2018) Histology of oogenesis and ovarian development in cultured Sepia esculenta. J Fish Sci China 25(3):503–511 (Chinese with English abstract)Google Scholar
- Young JZ (1971) The anatomy of the nervous system of Octopus vulgaris. Clarendon, OxfordGoogle Scholar