Advertisement

Expression and function analysis of a rice OsHSP40 gene under salt stress

  • Xin Wang
  • Huan Zhang
  • Lu-Yuan Shao
  • Xin Yan
  • Hui Peng
  • Jie-Xiu Ouyang
  • Shao-Bo Li
Research Article
  • 69 Downloads

Abstract

Heat shock proteins (HSPs) play essential roles in both plant growth and abiotic stress tolerance. In rice, OsHSP40 was recently reported to regulate programmed cell death (PCD) of suspension cells under high temperature. However, the expression and functions of OsHSP40 under normal growth or other abiotic stress conditions is still unknown. We reported the expression and function of a rice OsHSP40 gene under salt stress. Homologous proteins of OsHSP40 were collected from the NCBI database and constructed the neighbor-joining (NJ) phylogenetic tree. The expression pattern of OsHSP40 was detected by qRT-PCR under NaCl (150 mM) treatment. Then, identified a rice T-DNA insertion mutant oshsp40. At last, we compared and analyzed the phenotypes of oshsp40 and wild type under salt stress. OsHSP40 was a constitutively expressed small HSP (sHSP) gene and was close related to other plant sHSPs. Moreover, the expression of OsHSP40 was regulated by salt, varying across time points and tissues. Furthermore, the growth of T-DNA insertion mutant of OsHSP40 (designated as oshsp40) was suppressed by NaCl (150 mM) compared with that of the WT at seedling stage. Detailed measurement showed root and shoot length of the oshsp40 seedlings were significantly shorter than those of the WT seedlings under NaCl stress. In addition, the pot experiment results revealed that seedlings of oshsp40 withered more seriously compared with those of WT after NaCl treatment and recovery, and that survival rate and fresh weight of oshsp40 seedlings were significantly reduced. Taken together, these data suggested that OsHSP40 had multiple functions in rice normal growth and abiotic stress tolerance.

Keywords

OsHSP40 Oryza sativa Abiotic stress Expression pattern Function analysis 

Notes

Acknowledgements

This research was supported by grants from the National Natural Science Foundation of China (Nos. 31760080, 31460279, 31560383 and 31660296).

Author contributions

XW, JO and SL designed research; HZ and LS performed research; HP and XY did bioinformatic analysis and revised the manuscript; XW, JO and SL wrote the paper. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

13258_2018_749_MOESM1_ESM.tif (223 kb)
Fig. S1 Expression profile of OsHSP40 in different tissues of ZH11 based on microarray analysis. The scores are the average expression values obtained from microarrays. (TIF 223 KB)
13258_2018_749_MOESM2_ESM.docx (19 kb)
Supplementary material 2 (DOCX 18 KB)
13258_2018_749_MOESM3_ESM.docx (12 kb)
Supplementary material 3 (DOCX 11 KB)

References

  1. Asif MH, Lakhwani D, Pathak S, Bhambhani S, Bag SK, Trivedi PK (2014) Genome-wide identification and expression analysis of the mitogen-activated protein kinase gene family from banana suggest involvement of specific members in different stages of fruit ripening. Funct Integr Genom 14:161–175CrossRefGoogle Scholar
  2. Bernfur K, Rutsdottir G, Emanuelsson C (2017) The chloroplast-localized small heat shock protein Hsp21 associates with the thylakoid membranes in heat-stressed plants. Protein Sci 26(9):1773–1784CrossRefGoogle Scholar
  3. Cui J, Chen B, Wang HJ, Han Y, Chen X, Zhang W (2016) Glucosidase II β-subunit, a novel substrate for caspase-3-like activity in rice, plays as a molecular switch between autophagy and programmed cell death. Sci Rep 6:31764CrossRefGoogle Scholar
  4. Hsu JL, Wang LY, Wang SY, Lin CH, Ho KC, Shi FK, Chang IF (2009) Functional phosphoproteomic profiling of phosphorylation sites in membrane fractions of salt-stressed Arabidopsis thaliana. Proteome Sci 7:42CrossRefGoogle Scholar
  5. Jacob P, Hirt H, Bendahmane A (2017) The heat-shock protein/chaperone network and multiple stress resistance. Plant Biotechnol J 15(4):405–414CrossRefGoogle Scholar
  6. Joly AL, Wettstein G, Mignot G, Ghiringhelli F, Garrido C (2010) Dual role of heat shock proteins as regulators of apoptosis and innate immunity. J Innate Immun 2:238–247CrossRefGoogle Scholar
  7. Khush GS (2005) What it will take to feed 5.0 billion rice consumers in 2030. Plant Mol Biol 59:1–6CrossRefGoogle Scholar
  8. Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Peer YN, Rouze´ P, Rombauts S (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30(1):325–327CrossRefGoogle Scholar
  9. Li JJ, Li Y, Yin ZG, Jiang JH, Zhang MH, Guo X, Ye ZJ, Zhao Y, Xiong HY, Zhang ZY, Shao YJ, Jiang CH, Zhang HL, An G, Paek NC, Ali J, Li ZC (2017) OsASR5 enhances drought tolerance through a stomatal closure pathway associated with ABA and H2O2 signalling in rice. Plant Biotechnol J 15:183–196CrossRefGoogle Scholar
  10. Liao PF, Huang JQ, Tong PG, Nie W, Yan X, Feng YM, Peng H, Peng XJ, Li SB (2017) Characterization and expression analysis of inositolphosphorylceramide synthase family genes in rice (Oryza sativa L.). Genes Genomics 39:485–492CrossRefGoogle Scholar
  11. Liu JL, Luo MS, Yan X, Yu C, Li SB (2016) Characterization of genes coding for galacturonosyltransferaselike (GATL) proteins in rice. Genes Genomics 38:917–929CrossRefGoogle Scholar
  12. Mcloughlin F, Basha E, Fowler ME, Kim M, Bordowitz J, Katiyar-Aqarwal S, Vierling E (2016) Class I and II small heat shock proteins together with HSP101 protect protein translation factors during heat stress. Plant Physiol 172(2):1221–1236PubMedPubMedCentralGoogle Scholar
  13. Murakami T, Matsuba S, Funatsuki H, Kawaguchi K, Saruyama H, Tanida M, Sato Y (2004) Over-expression of a small heat shock protein, sHSP17.7, confers both heat tolerance and UV-B resistance to rice plants. Mol Breed 13:165–175CrossRefGoogle Scholar
  14. Ruibal C, Castro A, Carballo V, Szabados L, Vidal S (2013) Recovery from heat, salt and osmotic stress in Physcomitrella patens requires a functional small heat shock protein PpHsp16.4. BMC Plant Biol 13:174–191CrossRefGoogle Scholar
  15. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
  16. Sarkar NK, Kim YK, Grover A (2009) Rice sHsp genes: genomic organization and expression profiling under stress and development. BMC Genomics 10:393CrossRefGoogle Scholar
  17. Shen C, Que Z, Xia Y, Tang N, Li D, He R, Cao M (2017) Knock out of the annexin gene OsAnn3, via crispr/cas9-mediated genome editing decreased cold tolerance in rice. J Plant Biol 60:539–547CrossRefGoogle Scholar
  18. Sun W, Bernard C, Van De Cotte B, Van Montagu M, Verbruggen N (2001) At-HSP17.6A, encoding a small heat-shock protein in Arabidopsis, can enhance osmotolerance upon overexpression. Plant J 27:407–415CrossRefGoogle Scholar
  19. Sun W, Montagu MV, Verbruggen N (2002) Small heat shock proteins and stress tolerance in plants. Biochim Biophys Acta 1577:1–9CrossRefGoogle Scholar
  20. Tester M, Langridge P (2010) Breeding technologies to increase crop production in a changing world. Science 327:818–822CrossRefGoogle Scholar
  21. Tian L, Wang XY, Wang XP, Lei CL, Zhu F (2018) Starvation-, thermal- and heavy metal-associated expression of four small heat shock protein genes in musca domestica. Gene 642:268–276CrossRefGoogle Scholar
  22. Trent J (1996) A review of acquired thermotolerance, heat-shock proteins, and molecular chaperones in archaea. FEMS Microbiol Rev 18:249–258CrossRefGoogle Scholar
  23. Venkatesh J, Park SW (2015) Genome-wide analysis and expression profiling of DNA-binding with one zinc finger (Dof) transcription factor family in potato. Plant Physiol Biochem 94:73–85CrossRefGoogle Scholar
  24. Vermeulen S, Zougmore R, Wollenberg E, Thornton P, Nelson G, Kristjanson P, Kinyangi J, Jarvis A, Hansen J, Challinor A, Acmpbell B, Aggarwal P (2012) Climate change, agriculture and food security: a global partnership to link research and action for low-income agricultural producers and consumers. Curr Opin Environ Sustain 4:128–133CrossRefGoogle Scholar
  25. Vierling E (1991) The roles of heat shock proteins in plants. Annu Rev Plant Biol 42:579–620CrossRefGoogle Scholar
  26. Wang YS, An CY, Zhang XD, Yao JQ, Zhang YP, Sun YJ, Yu FH, Amador DM, Mou ZL (2013) The Arabidopsis elongator complex subunit2 epigenetically regulates plant immune responses. Plant Cell 25:762–776CrossRefGoogle Scholar
  27. Wang X, Zhou W, Lu ZH, Ouyang YD, O CS, Yao JL (2015a) A lipid transfer protein, OsLTPL36, is essential for seed development and seed quality in rice. Plant Sci 239:200–208CrossRefGoogle Scholar
  28. Wang AQ, Yu XH, Mao Y, Liu Y, Liu GQ, Liu YS, Niu XL (2015b) Overexpression of a small heat-shock-protein gene enhances tolerance to abiotic stresses in rice. Plant Breed 134(4):384–393CrossRefGoogle Scholar
  29. Wang D, Qu ZP, Yang L, Zhang QZ, Liu ZH, Do T, Adelson DL, Wang ZY, Searle I, Zhu JK (2017) Transposable elements (TEs) contribute to stress-related long intergenic noncoding RNAs in plants. Plant J 90:133–146CrossRefGoogle Scholar
  30. Xue LJ, Zhang JJ, Xue HW (2009) Characterization and expression profiles of miRNAs in rice seeds. Nucleic Acids Res 37(3):916–930CrossRefGoogle Scholar
  31. Ye SF, Yu SW, Shu LB, Wu JH, Wu AZ, Luo LJ (2012) Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice. Chin Sci Bull 57(4):336–343CrossRefGoogle Scholar
  32. Yi J, An G (2013) Utilization of T-DNA tagging lines in rice. J Plant Biol 56(2):85–90CrossRefGoogle Scholar
  33. Zhai M, Sun Y, Jia C, Peng S, Liu Z, Yang G (2016) Over-expression of JrsHSP17.3 gene from Juglans regia confer the tolerance to abnormal temperature and NaCl stresses. J Plant Biol 59:549–558CrossRefGoogle Scholar
  34. Zhang Q (2007) Strategies for developing Green Super Rice. Proc Natl Acad Sci USA 104:16402–16409CrossRefGoogle Scholar

Copyright information

© The Genetics Society of Korea and Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, School of Life SciencesNanchang UniversityNanchangChina
  2. 2.Medical Laboratory Education CenterNanchang UniversityNanchangChina
  3. 3.College of Life SciencesGuangxi Normal UniversityGuilinChina
  4. 4.Hunan Hi-Tech Bio-Agro Co., LtdYueyangChina

Personalised recommendations