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Genes & Genomics

, Volume 41, Issue 1, pp 107–111 | Cite as

Study on the bio-function of lipA gene in Aspergillus flavus

  • Wenzhao Bai
  • Tiejun Feng
  • Faxiu Lan
  • Guanglan Lin
  • Yu Li
  • Opemipo Esther Fasoyin
  • Yaju Liu
  • Kunzhi JiaEmail author
Research Article
  • 70 Downloads

Abstract

Lipoic acid synthase (LipA) plays a role in lipoic acid synthesis and potentially affects the levels of acetyl-CoA, the critical precursor of tricarboxylic acid (TCA) cycle. Considering the potential effect of LipA on TCA cycle, whether the enzyme is involved in the growth and aflatoxin B1 (AFB1) biosynthesis, the significant events in Aspergillus flavus is yet known. The study was designed to explore the role of lipA gene in A. flavus, including growth rate, conidiation, sclerotia formation, and biosynthesis of AFB1. LipA coding lipoic acid synthetase was knocked out using homologous recombination. The role of lipA gene in A. flavus morphogenesis (including colony size, conidiation, and sclerotia formation) was explored on various media, and the bio-function of lipA gene in the biosynthesis of AFB1 was analyzed by thin layer chromatography analysis. The growth was suppressed in △lipA. The formation of conidia and sclerotia was also reduced when lipA gene was deleted. Moreover, AFB1 was down-regulated in ΔlipA compared with WT controls. LipA plays a role in the development of A. flavus and AFB1 biosynthesis, contributing to the full understanding of the lipA bio-function in A. flavus.

Keywords

Aspergillus flavus Lipoic acid synthetase lipA Aflatoxin B1 

Notes

Acknowledgements

This work was supported by the Program for Cultivation of Outstanding Youth Science and Technology Talents at Fujian Agriculture and Forestry University (xjq201410), the science & technology innovation fund of Fujian Agriculture and Forestry University (KFA17583A), the Fujian Provincial Nature Science Foundation (2015J05052), and Agricultural Five-new Engineering Projects of Fujian Development and Reform Commission.

References

  1. Bennett JW, Klich M (2003) Mycotoxins. Clin Microbiol Rev 16:497–516CrossRefGoogle Scholar
  2. Chang PK, Scharfenstein LL, Wei Q, Bhatnagar D (2010) Development and refinement of a high-efficiency gene-targeting system for Aspergillus flavus. J Microbiol Methods 81(3):240–246CrossRefGoogle Scholar
  3. Cronan JE (2014) Biotin and lipoic acid: synthesis, attachment, and regulation, EcoSal Plus.  https://doi.org/10.1128/ecosalplus.ESP-0001-2012 Google Scholar
  4. Frey PA, Hegeman AD, Ruzicka FJ (2008) The radical SAM superfamily. Crit Rev Biochem Mol Biol 43(1):63–88CrossRefGoogle Scholar
  5. Herbert AA, Guest JR (1968) Biochemical and genetic studies with lysine + methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J Gen Microbiol 53:363–381CrossRefGoogle Scholar
  6. Li Y, He Y, Li X, Fasoyin OE, Hu Y, Liu Y, Yuan J, Zhuan Z, Wang S (2017) Histone methyltransferase aflrmetA gene is involved in the morphogenesis, mycotoxin biosynthesis, and pathogenicity of Aspergillus flavus. Toxicon 127:112–121CrossRefGoogle Scholar
  7. Liang L, Liu Y, Yang K, Lin G, Xu Z, Lan H, Wang X, Wang S (2017) The putative histone methyltransferase DOT1 regulates aflatoxin and pathogenicity attributes in Aspergillus flavus. Toxins 9:232CrossRefGoogle Scholar
  8. Nie Y, Yu S, Qiu M, Wang X, Wang Y, Bai Y, Zhang F, Wang S (2016) Aspergillus flavus SUMO contributes to fungal virulence and toxin attributes. J Agric Food Chem 64:6772–6782CrossRefGoogle Scholar
  9. Packer L, Witt EH, Tritschler HJ (1995) alpha-Lipoic acid as a biological antioxidant. Free Radical Biol Med 19:227–250CrossRefGoogle Scholar
  10. Perrone G, Susca A, Cozzi G, Ehrlich K, Varga J, Frisvad JC, Meijer M, Noonim P, Mahakarnchanakul W, Samson RA (2007) Biodiversity of Aspergillus species in some important agricultural products. Stud Mycol 59:53–66CrossRefGoogle Scholar
  11. Rao JP, Subramanyam C (2000) Calmodulin mediated activation of acetyl-CoA carboxylase during aflatoxin production by Aspergillus parasiticus. Lett Appl Microbiol 4:277–281Google Scholar
  12. Sen CK, Packer L (1996) Antioxidant and redox regulation of gene transcription. FASEB J 10:709–720CrossRefGoogle Scholar
  13. Singh VK, Mangalam AK, Dwivedi S, Naik S (1998) Primer premier: program for design of degenerate primers from a protein sequence. Biotechniques 24(2):318–319CrossRefGoogle Scholar
  14. Yang K, Liang L, Ran F, Liu Y, Li Z, Lan H, Gao P, Zhuang Z, Zhang F, Nie X, Yirga SK, Wang S (2016) The DmtA methyltransferase contributes to Aspergillus flavus conidiation, sclerotial production, aflatoxin biosynthesis and virulence. Sci Rep 6:23259.  https://doi.org/10.1038/srep23259 CrossRefGoogle Scholar
  15. Zhuang ZH, Li H, Yang JN, Liu X, Gao YY, Li QF, Wang SY, Peng XX (2011) Gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri. Fish Shellfish Immunol 30(2):700–705CrossRefGoogle Scholar
  16. Zhuang ZH, Lohmar JM, Satterlee T, Cary JW, Calvo AM (2016) The master transcription factor mtfA governs Aflatoxin production, morphological development and pathogenicity in the fungus Aspergillus flavus, Toxins 8(1):E29.  https://doi.org/10.3390/toxins8010029 CrossRefGoogle Scholar

Copyright information

© The Genetics Society of Korea and Springer Nature B.V. 2018

Authors and Affiliations

  • Wenzhao Bai
    • 1
  • Tiejun Feng
    • 2
  • Faxiu Lan
    • 2
  • Guanglan Lin
    • 2
  • Yu Li
    • 2
  • Opemipo Esther Fasoyin
    • 2
  • Yaju Liu
    • 2
  • Kunzhi Jia
    • 2
    Email author
  1. 1.College of Biological Science and EngineeringShaanxi University of TechnologyHanzhongChina
  2. 2.School of Life SciencesFujian Agriculture and Forestry UniversityFuzhouChina

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