Study on the bio-function of lipA gene in Aspergillus flavus
- 70 Downloads
Lipoic acid synthase (LipA) plays a role in lipoic acid synthesis and potentially affects the levels of acetyl-CoA, the critical precursor of tricarboxylic acid (TCA) cycle. Considering the potential effect of LipA on TCA cycle, whether the enzyme is involved in the growth and aflatoxin B1 (AFB1) biosynthesis, the significant events in Aspergillus flavus is yet known. The study was designed to explore the role of lipA gene in A. flavus, including growth rate, conidiation, sclerotia formation, and biosynthesis of AFB1. LipA coding lipoic acid synthetase was knocked out using homologous recombination. The role of lipA gene in A. flavus morphogenesis (including colony size, conidiation, and sclerotia formation) was explored on various media, and the bio-function of lipA gene in the biosynthesis of AFB1 was analyzed by thin layer chromatography analysis. The growth was suppressed in △lipA. The formation of conidia and sclerotia was also reduced when lipA gene was deleted. Moreover, AFB1 was down-regulated in ΔlipA compared with WT controls. LipA plays a role in the development of A. flavus and AFB1 biosynthesis, contributing to the full understanding of the lipA bio-function in A. flavus.
KeywordsAspergillus flavus Lipoic acid synthetase lipA Aflatoxin B1
This work was supported by the Program for Cultivation of Outstanding Youth Science and Technology Talents at Fujian Agriculture and Forestry University (xjq201410), the science & technology innovation fund of Fujian Agriculture and Forestry University (KFA17583A), the Fujian Provincial Nature Science Foundation (2015J05052), and Agricultural Five-new Engineering Projects of Fujian Development and Reform Commission.
- Rao JP, Subramanyam C (2000) Calmodulin mediated activation of acetyl-CoA carboxylase during aflatoxin production by Aspergillus parasiticus. Lett Appl Microbiol 4:277–281Google Scholar
- Yang K, Liang L, Ran F, Liu Y, Li Z, Lan H, Gao P, Zhuang Z, Zhang F, Nie X, Yirga SK, Wang S (2016) The DmtA methyltransferase contributes to Aspergillus flavus conidiation, sclerotial production, aflatoxin biosynthesis and virulence. Sci Rep 6:23259. https://doi.org/10.1038/srep23259 CrossRefGoogle Scholar