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Transcriptome profiling and identification of functional genes involved in H2S response in grapevine tissue cultured plantlets

  • Qian Ma
  • Jingli Yang
Research Article
  • 122 Downloads

Abstract

Hydrogen sulfide (H2S), a small bioactive gas, has been proved functioning in plant growth and development as well as alleviation of abiotic stresses, which including promoting seed germination, accelerating embryonic root growth, regulating flower senescence, inducing stomatal closure, and defending drought, heat, heavy metals and osmotic stresses etc. However, the molecular functioning mechanism of H2S was still unclear. The primary objective of this research was to analyze the transcriptional differences and functional genes involved in the H2S responses. In details, 4-week-old plantlets in tissue culture of grapevine (Vitis vinifera L.) cultivar ‘Zuoyouhong’ were sprayed with 0.1 mM NaHS for 12 h, and then transcriptome sequencing and qRT-PCR analysis were used to study the transcriptional differences and functional genes involved in the H2S responses. Our results indicated that 650 genes were differentially expressed after H2S treatment, in which 224 genes were up-regulated and 426 genes were down-regulated. The GO enrichment analysis and KEGG enrichment analysis results indicated that the up-regulated genes after H2S treatment focused on carbon metabolism, biosynthesis of amino acids, and glycolysis/gluconeogenesis, and the down-regulated genes were mainly in metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction. Analyzing the transcription factor coding genes in details, it was indicated that 10 AP2/EREBPs, 5 NACs, 3 WRKYs, 3 MYBs, and 2 bHLHs etc. transcription factor coding genes were up-regulated, while 4 MYBs, 3 OFPs, 3 bHLHs, 2 AP2/EREBPs, 2 HBs etc. transcription factor coding genes were down-regulated. Taken together, H2S increased the productions in secondary metabolites and a variety of defensive compounds to improve plant development and abiotic resistance, and extend fruits postharvest shelf life by regulating the expression of AP2/EREBPs, WRKYs, MYBs, CABs, GRIP22, FERRITINs, TPSs, UGTs, and GHs etc.

Keywords

H2Transcriptome Sequencing Vitis vinifera 

Notes

Acknowledgements

This work was supported by Science and Technology Project of Higher Education in Shandong Province (Grant No. J14LE12), Research Foundation for Advanced Talents of Qingdao Agricultural University, and National Natural Science Foundation of China (Grant No. 31540090).

Compliance with ethical standards

Conflict of interest

We declared that the authors of this paper have no conflict of interest.

Ethical approval

This article does not contain any studies with human subjects or animals performed by any of the authors.

Supplementary material

13258_2018_723_MOESM1_ESM.xls (40.2 mb)
Supplementary material 1: The grapevine gene annotation information in this research. (XLS 41196 KB)
13258_2018_723_MOESM2_ESM.docx (25 kb)
Supplementary material 2: The sequence templates used in qRT-PCR. (DOCX 25 KB)
13258_2018_723_MOESM3_ESM.docx (20 kb)
Supplementary material 3: Oligonucleotide primers used in this study. (DOCX 19 KB)
13258_2018_723_MOESM4_ESM.tif (90 kb)
Supplementary material 4: The FPKM density distribution of H2S treated (H2S) and untreated grapevine plantlets in tissue culture (CTR). In figure, the X-axis number was calculated by log10(FPKM+1), and the Y-axis represented the gene density. (TIF 89 KB)
13258_2018_723_MOESM5_ESM.tif (90 kb)
Supplementary material 5: The volcano plot of differentially expressed genes with adjusted p-value <0.05 after H2S treatment. The X-axis indicated the log fold change of genes after H2S treatment, and the Y-axis showed the significant differences of gene expression. The highly expressed genes were represented by red spots, and the lowly regulated genes were denoted by green spots in the figure. (TIF 90 KB)
13258_2018_723_MOESM6_ESM.tif (97 kb)
Supplementary material 6: The volcano plot of differentially expressed genes with adjusted p-value <0.05, fold change >2, and the average FPKM >1 after H2S treatment. The highly expressed genes were panted by red color, and the lowly regulated genes were painted by green. (TIF 97 KB)
13258_2018_723_MOESM7_ESM.xls (34 kb)
Supplementary material 7: The up-regulated genes after H2S treatment. (XLS 34 KB)
13258_2018_723_MOESM8_ESM.xls (66 kb)
Supplementary material 8: The down-regulated genes after H2S treatment. (XLS 66 KB)
13258_2018_723_MOESM9_ESM.xls (189 kb)
Supplementary material 9: The GO annotation of up-regulated DEGs used for functional enrichment analysis. (XLS 189 KB)
13258_2018_723_MOESM10_ESM.xls (59 kb)
Supplementary material 10: The KEGG annotation of up-regulated DEGs used for functional enrichment analysis. (XLS 58 KB)
13258_2018_723_MOESM11_ESM.xls (456 kb)
Supplementary material 11: The GO annotation of down-regulated DEGs used for functional enrichment analysis. (XLS 456 KB)
13258_2018_723_MOESM12_ESM.xls (55 kb)
Supplementary material 12: The KEGG annotation of down-regulated DEGs used for functional enrichment analysis. (XLS 54 KB)

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Copyright information

© The Genetics Society of Korea and Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Shandong Key Laboratory of Plant Biotechnology, College of Life SciencesQingdao Agricultural UniversityQingdaoPeople’s Republic of China

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