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Genes & Genomics

, Volume 40, Issue 11, pp 1199–1211 | Cite as

Low pH stress responsive transcriptome of seedling roots in wheat (Triticum aestivum L.)

  • Haiyan Hu
  • Jie He
  • Junjie Zhao
  • Xingqi Ou
  • Hongmin Li
  • Zhengang Ru
Research Article

Abstract

Soil acidification is one of major problems limiting crop growth and especially becoming increasingly serious in China owing to excessive use of nitrogen fertilizer. Only the STOP1 of Arabidopsis was identified clearly sensitive to proton rhizotoxicity and the molecular mechanism for proton toxicity tolerance of plants is still poorly understood. The main objective of this study was to investigate the transcriptomic change in plants under the low pH stress. The low pH as a single factor was employed to induce the response of the wheat seedling roots. Wheat cDNA microarray was used to identify differentially expressed genes (DEGs). A total of 1057 DEGs were identified, of which 761 genes were up-regulated and 296 were down-regulated. The greater percentage of up-regulated genes involved in developmental processes, immune system processes, multi-organism processes, positive regulation of biological processes and metabolic processes of the biological processes. The more proportion of down-regulation genes belong to the molecular function category including transporter activity, antioxidant activity and molecular transducer activity and to the extracellular region of the cellular components category. Moreover, most genes among 41 genes involved in ion binding, 17 WAKY transcription factor genes and 17 genes related to transport activity were up-regulated. KEGG analysis showed that the jasmonate signal transduction and flavonoid biosynthesis might play important roles in response to the low pH stress in wheat seedling roots. Based on the data, it is can be deduced that WRKY transcription factors might play a critical role in the transcriptional regulation, and the alkalifying of the rhizosphere might be the earliest response process to low pH stress in wheat seedling roots. These results provide a basis to reveal the molecular mechanism of proton toxicity tolerance in plants.

Keywords

Acid soil Low pH stress Resistance genes Transcriptome Triticum aestivum L. 

Notes

Acknowledgements

This study was funded by Science and Technology Program of Henan Province, China (Grant No. 142102110041). Project supported by the State Key Development Program for Research of China (Grant No. 2017YFD0100705).

Compliance with ethical standards

Conflict of interest

Haiyan Hu, Jie He, Junjie Zhao, Xingqi Ou, Hongmin Li and Zhengang Ru declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human subjects or animals performed by any of the authors.

Supplementary material

13258_2018_680_MOESM1_ESM.docx (26 kb)
Supplementary material 1 (DOCX 25 KB)

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Copyright information

© The Genetics Society of Korea and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Life Science and TechnologyHenan Institute of Science and TechnologyXinxiangChina
  2. 2.Collaborative Innovation Center of Modern Biological BreedingXinxiangChina
  3. 3.Henan Engineering Research Center of Crop Genome EditingXinxiangChina

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