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Genes & Genomics

, Volume 40, Issue 11, pp 1119–1125 | Cite as

Association between expression levels and growth trait-related SNPs located in promoters of the MC4R and MSTN genes in Spinibarbus hollandi

  • Yang Yang
  • Zhaojun Lan
  • Hu Shu
  • Huiqiang Zhou
  • Xiaolu Jiang
  • Liping Hou
  • Pinghua Gu
Research Article
  • 150 Downloads

Abstract

Melanocortin 4 receptor: (MC4R) and Myostatin (MSTN) are two important growth trait-related genes in animals. In this study, we showed that two SNPs, MC4R-719A>G and MSTN-519C>T, found in the promoters of the MC4R and MSTN genes, respectively, are both associated with growth traits in Spinibarbus hollandi. Furthermore, we observed that there were significant associations between the expression levels of the MC4R and MSTN genes and these two growth trait-related SNPs. The expression level of MC4R gene in brain was lower in GG genotype fish with extremely high growth performance than that in AA genotype fish with extremely low growth performance. Expression level of the MSTN gene in muscle was lower in TT genotype fish with extremely high growth performance than that in CC and CT genotype fish with lower growth performance. The results indicated that these SNPs located in the promoters of MC4R and MSTN are associated with growth-related traits through modification of gene expression levels. The MSTN and MC4R SNPs may have useful application in effective marker-assisted selection aimed to increase output in S. hollandi.

Keywords

Melanocortin-4 receptor (MC4RMyostatin (MSTNExpression level Spinibarbus hollandi Single-nucleotide polymorphism (SNP) 

Notes

Acknowledgements

This work is supported by the National public industry major projects of China (No. 201303048) and the Guangdong Provincial Science and Technology Program (No. 2014A020208145).

Compliance with ethical standards

Conflict of interest

Yang Yang declares that he does not have conflict of interest. Zhaojun LAN declares that he does not have conflict of interest. Hu Shu declares that he does not have conflict of interest. Huiqiang Zhou declares that he does not have conflict of interest. Xiaolu Jiang declares that she does not have conflict of interest. Liping Hou declares that she does not have conflict of interest. Pinghua Gu declares that he does not have conflict of interest.

Research involving human and animal rights

All animal experiments throughout the study were conducted in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals for the Science and Technology Bureau of China.

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Copyright information

© The Genetics Society of Korea and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Life ScienceGuangzhou UniversityGuangzhouChina
  2. 2.Shaoguan Fisheries Research InstituteShaoguanChina

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