Association between expression levels and growth trait-related SNPs located in promoters of the MC4R and MSTN genes in Spinibarbus hollandi
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Melanocortin 4 receptor: (MC4R) and Myostatin (MSTN) are two important growth trait-related genes in animals. In this study, we showed that two SNPs, MC4R-719A>G and MSTN-519C>T, found in the promoters of the MC4R and MSTN genes, respectively, are both associated with growth traits in Spinibarbus hollandi. Furthermore, we observed that there were significant associations between the expression levels of the MC4R and MSTN genes and these two growth trait-related SNPs. The expression level of MC4R gene in brain was lower in GG genotype fish with extremely high growth performance than that in AA genotype fish with extremely low growth performance. Expression level of the MSTN gene in muscle was lower in TT genotype fish with extremely high growth performance than that in CC and CT genotype fish with lower growth performance. The results indicated that these SNPs located in the promoters of MC4R and MSTN are associated with growth-related traits through modification of gene expression levels. The MSTN and MC4R SNPs may have useful application in effective marker-assisted selection aimed to increase output in S. hollandi.
KeywordsMelanocortin-4 receptor (MC4R) Myostatin (MSTN) Expression level Spinibarbus hollandi Single-nucleotide polymorphism (SNP)
This work is supported by the National public industry major projects of China (No. 201303048) and the Guangdong Provincial Science and Technology Program (No. 2014A020208145).
Compliance with ethical standards
Conflict of interest
Yang Yang declares that he does not have conflict of interest. Zhaojun LAN declares that he does not have conflict of interest. Hu Shu declares that he does not have conflict of interest. Huiqiang Zhou declares that he does not have conflict of interest. Xiaolu Jiang declares that she does not have conflict of interest. Liping Hou declares that she does not have conflict of interest. Pinghua Gu declares that he does not have conflict of interest.
Research involving human and animal rights
All animal experiments throughout the study were conducted in accordance with the Regulations for the Administration of Affairs Concerning Experimental Animals for the Science and Technology Bureau of China.
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