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Expression analysis of miR-221-3p and its target genes in horses

  • So-Won Kim
  • Ara Jo
  • Jennifer Im
  • Hee-Eun Lee
  • Heui-Soo KimEmail author
Research Article
  • 19 Downloads

Abstract

Background

A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses (Equus caballus) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution.

Objectives

We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues.

Methods

We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR).

Results

Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene.

Conclusion

Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.

Keywords

Equus caballus Micro RNA 221-3p CDKN1C Quantitative polymerase chain reaction (qPCR) Expression analysis 

Notes

Acknowledgements

This work was supported by a grant from the Ministry of Education, Republic of Korea (NRF-2015R1D1A1A01056650).

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflicts of interest.

Ethical approval

The animal protocol and sample extraction method in this study were reviewed by the Pusan National University-Institutional Animal Care and Use Committee (PNU-IACUC) for the ethical procedures and scientific care (approval number PNU-2013-0411).

Research involving human participants

This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© The Genetics Society of Korea 2019

Authors and Affiliations

  1. 1.Department of Biological Sciences, College of Natural SciencesPusan National UniversityBusanRepublic of Korea
  2. 2.Institute of Systems BiologyPusan National UniversityBusanRepublic of Korea

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