Temperature-dependent immune response of olive flounder (Paralichthys olivaceus) infected with viral hemorrhagic septicemia virus (VHSV)
Olive flounder (Paralichthys olivaceus) is one of the most economically important aquaculture fish. However, its production is often affected by various diseases, especially viral hemorrhagic septicemia virus (VHSV) that cause serious economic losses. In this study, we sequenced the whole transcriptome of the P. olivaceus using Illumina RNA-sEq. De novo assembly of control and virus-infected cDNA libraries of head kidney at 13 and 20 °C was accomplished with 2,007,532,438 raw reads, resulting in 244,578 unigenes with an average length of 533 bp and found 65,535 candidate coding unigenes with homology to other species by BLAST analysis. DEG analysis among control and virus-infected head kidney samples of 13 and 20 °C revealed that 1290 up-regulated and 162 down-regulated genes (p ≤ 0.01), linked to metabolism, virulence factors, adhesion and immune-response. We constructed an expressed gene catalog for the P. olivaceus to serve as a resource for marine environmental genomic and immuno-genetic/genomic studies focused on uncovering the molecular mechanisms underlying the responses of P. olivaceus to VHSV under different temperature.
KeywordsParalichthys olivaceus Olive flounder Transcriptome RNA-Seq Viral hemorrhagic septicemia virus (VHSV) Head kidney
JYH, KM, KH, MGK, and JHP conceived and designed the study and wrote the paper. JSS, SIY, SDH, BYJ, and MHS performed the experiments and data analysis. All authors read and approved the final manuscript.
This work was supported by a grant from the National Institute of Fisheries Science in the Republic of Korea (Grant Number: R2017067).
Compliance with ethical standards
Conflict of interest
Jee Youn Hwang, Kesavan Markkandan, Kyudong Han, Mun Gyeong Kwon, Jung Soo Seo, Seung-il Yoo, Seong Don Hwang, Bo Young Ji, Maeng-Hyun Son, and Jun-hyung Park declare that they do not have conflict of interest.
As olive flounder is not an endangered or protected species, and collections were only made from commercial fish farm and reared in National Institute of Fisheries Science (NIFS), South Korea which is a government funded research institute. Hence, no specific permits were required for the described study.
- Pereiro P, Balseiro P, Romero A, Dios S, Forn-Cuni G, Fuste B, Planas JV, Beltran S, Novoa B, Figueras A (2012) High-throughput sequence analysis of turbot (Scophthalmus maximus) transcriptome using 454-pyrosequencing for the discovery of antiviral immune genes. PLoS ONE 7:e35369CrossRefPubMedPubMedCentralGoogle Scholar
- Wang Z, Liu C, Niu J, Yang F, Wu X, Qi J (2016) Transcription of dynein heavy chain 9 (DNAH9) in Paralichthys olivaceus: insights into the evolution of the DNAH9 gene. Pak J Zool 48:1331–1335Google Scholar