Genes & Genomics

, Volume 39, Issue 10, pp 1157–1162 | Cite as

Long interspersed element-1 open reading frame 1 protein expression profiles in ovarian cancers

  • Ka Yeong Yun
  • Eun Ji Ko
  • Heung Yeol Kim
  • Ji Young Lee
  • Wan Kyu Eo
  • Mee Sun Ock
  • Heui-Soo Kim
  • Ki Hyung KimEmail author
  • Hee-Jae ChaEmail author
Research Article


Long interspersed element-1 (LINE-1) retrotransposons are autonomous mobile DNA elements with unique activity that account for about one-fifth of the human genome. Recently, it has been reported that the expression of LINE-1 is closely related to cancer prognosis, and LINE-1 hypomethylation might contribute to the acquisition of aggressive tumor behavior. Despite the importance of LINE-1 expression in cancers, research on the expression of LINE-1 open reading frame (ORF) proteins is very limited. Here, we investigated the expression profiles of LINE-1 ORF1p in ovarian cancer tissue microarrays containing 100 surgical specimens including adjacent normal ovary tissue, primary ovarian cancers, and metastatic ovarian cancers in lymph node. The tissue microarray was stained with mouse monoclonal antibody to LINE-1 ORFp1 for immunofluorescence analysis, and expression levels were evaluated by image analysis. LINE-1 ORFp was significantly overexpressed in ovarian cancers compared with normal tissues and especially upregulated in metastatic ovarian cancers. In addition, the expression of LINE-1 ORF1p was significantly higher in older ovarian cancer patients compared with young patients. These results indicate that expression of LINE-1 ORF1p is related to the progression of ovarian cancers and, in particular, to the age of the patient and the metastatic potential of the cancer.


LINE-1 ORFp1 Protein expression Ovarian cancer 



This study was supported by Biomedical Research Institute Grant (2015-22), Pusan National University Hospital.

Compliance with ethical standards

Conflict of interest

Ka Yeong Yun declares that he has no conflict of interest. Eun Ji Ko declares that he has no conflict of interest. Heung Yeol Kim declares that he has no conflict of interest. Ji Young Lee declares that he has no conflict of interest. Wan Kyu Eo declares that he has no conflict of interest. Mee Sun Ock declares that he has no conflict of interest. Heui-Soo Kim declares that he has no conflict of interest. Ki Hyung Kim declares that he has no conflict of interest. Hee-Jae Cha declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

13258_2017_589_MOESM1_ESM.docx (26 kb)
Supplementary material 1 (DOCX 25 KB)


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Copyright information

© The Genetics Society of Korea and Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Department of MedicinePusan National University School of MedicineBusanRepublic of Korea
  2. 2.Department of Parasitology and GeneticsKosin University College of MedicineBusanRepublic of Korea
  3. 3.Department of Obstetrics and GynecologyKosin University College of MedicineBusanRepublic of Korea
  4. 4.Department of Obstetrics and GynecologyKonkuk University School of MedicineSeoulRepublic of Korea
  5. 5.Department of Internal MedicineKyung Hee UniversitySeoulRepublic of Korea
  6. 6.Department of Biological SciencesPusan National UniversityBusanRepublic of Korea
  7. 7.Department of Obstetrics and GynecologyPusan National University School of MedicineBusanRepublic of Korea
  8. 8.Biomedical Research Institute and Pusan Cancer CenterPusan National University HospitalBusanRepublic of Korea

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