Genes & Genomics

, Volume 38, Issue 1, pp 13–23 | Cite as

Correlation between the DNA methyltransferase (Dnmt) gene family and genome-wide 5-methylcytosine (5mC) in rotifer, copepod, and fish

Research Article

Abstract

DNA methyltransferases (DNMTs) are an enzyme family that catalyzes the transfer of a methyl group to DNA for a wide variety of biological functions. To determine whether the number and composition of the Dnmt gene family affects the 5-methylcytosine (5mc) ratio at the genome level, genome-wide 5mc ratios from three marine animals, the mangrove killifish (Kryptolebias marmoratus), an intertidal copepod (Tigriopus japonicus), and a monogonont rotifer (Brachionus koreanus), were analyzed in each organism after the cloning of Dnmt genes. Lineage- and teleost-specific gene evolution was observed in the vertebrate Dnmt3 gene family, while unique gene expansion was found in the T. japonicusDnmt1 gene family. However, the rotifer did not have any apparent homologue of Dnmt1 or Dnmt3 in its genome. This gene information was highly supportive of genome-wide 5mc levels in the three marine animals. Therefore, the absence or presence of the Dnmt gene family could be an important evolutionary parameter of how this could affect genome-wide epigenetic metabolism.

Keywords

DNA methyltransferase Dnmt 5-methylcytosine 5mc Kryptolebias marmoratus Tigriopus japonicus Brachionus koreanus 

Notes

Acknowledgments

We thank three anonymous reviewers for their helpful comments and also thank Prof. Hans-U. Dahms for his comments on the revised manuscript. This work was supported by a grant by the Marine Biotechnology Program (PJT200620, Genome analysis of marine organisms and development of functional application) funded by the Ministry of Oceans and Fisheries, Korea, to Jae-Seong Lee.

Compliance with ethical standards

Compliance of bioethics

The fish were reared in accordance with the Animal Welfare Ethical Committee of the Sungkyunkwan University.

Supplementary material

13258_2015_333_MOESM1_ESM.docx (20 kb)
Supplementary material 1 (DOCX 20 kb)
13258_2015_333_MOESM2_ESM.pptx (560 kb)
Supplementary material 2 (PPTX 559 kb)

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Copyright information

© The Genetics Society of Korea and Springer-Science and Media 2015

Authors and Affiliations

  1. 1.Department of Biological Science, College of ScienceSungkyunkwan UniversitySuwonSouth Korea
  2. 2.School of BiosciencesUniversity of BirminghamBirminghamUK
  3. 3.Department of ToxicologyUniversity of WürzburgWürzburgGermany
  4. 4.Department of Marine Science, College of Natural SciencesIncheon National UniversityIncheonSouth Korea

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