Correlation between the DNA methyltransferase (Dnmt) gene family and genome-wide 5-methylcytosine (5mC) in rotifer, copepod, and fish
- 398 Downloads
DNA methyltransferases (DNMTs) are an enzyme family that catalyzes the transfer of a methyl group to DNA for a wide variety of biological functions. To determine whether the number and composition of the Dnmt gene family affects the 5-methylcytosine (5mc) ratio at the genome level, genome-wide 5mc ratios from three marine animals, the mangrove killifish (Kryptolebias marmoratus), an intertidal copepod (Tigriopus japonicus), and a monogonont rotifer (Brachionus koreanus), were analyzed in each organism after the cloning of Dnmt genes. Lineage- and teleost-specific gene evolution was observed in the vertebrate Dnmt3 gene family, while unique gene expansion was found in the T. japonicus Dnmt1 gene family. However, the rotifer did not have any apparent homologue of Dnmt1 or Dnmt3 in its genome. This gene information was highly supportive of genome-wide 5mc levels in the three marine animals. Therefore, the absence or presence of the Dnmt gene family could be an important evolutionary parameter of how this could affect genome-wide epigenetic metabolism.
KeywordsDNA methyltransferase Dnmt 5-methylcytosine 5mc Kryptolebias marmoratus Tigriopus japonicus Brachionus koreanus
We thank three anonymous reviewers for their helpful comments and also thank Prof. Hans-U. Dahms for his comments on the revised manuscript. This work was supported by a grant by the Marine Biotechnology Program (PJT200620, Genome analysis of marine organisms and development of functional application) funded by the Ministry of Oceans and Fisheries, Korea, to Jae-Seong Lee.
Compliance with ethical standards
Compliance of bioethics
The fish were reared in accordance with the Animal Welfare Ethical Committee of the Sungkyunkwan University.
- Gao F, Liu X, Wu XP, Wang XL, Gong D, Lu H, Xia Y, Song Y, Wang J, Du J, Liu S, Han X, Tang Y, Yang H, Jin Q, Zhang X, Liu M (2012) Differential DNA methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis. Genome Biol 13:R100CrossRefPubMedPubMedCentralGoogle Scholar
- Lee B-Y, Kim H-S, Hwang D-S, Won E-J, Choi B-S, Choi I-Y, Park HG, Rhee J-S, Lee J-S (2015) Whole transcriptome analysis of the monogonont rotifer Brachionus koreanus provides molecular resources for developing biomarkers of carbohydrate metabolism. Comp Biochem Physiol D 14:33–41Google Scholar
- Vandegehuchte MB, Lemière F, Janssen CR (2009) Quantitative DNA-methylation in Daphnia magna and effects of multigeneration Zn exposure. Comp Biochem Physiol C 150:343–348Google Scholar