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Genes & Genomics

, Volume 37, Issue 4, pp 387–397 | Cite as

Gene expression profile affected by volatiles of new plant growth promoting rhizobacteria, Bacillus subtilis strain JS, in tobacco

  • Ji-Seong Kim
  • Jeongeun Lee
  • Sang-Gyu Seo
  • Chanhui Lee
  • Su Young Woo
  • Sun-Hyung Kim
Research Article

Abstract

Specific bacterial strains that promote plant growth have been investigated extensively and are characterized as plant growth-promoting rhizobacteria (PGPR). To identify the series of genes involved in PGPR-mediated enhanced plant growth, suppression subtractive hybridization was performed using cDNA from tobacco plants exposed to the PGPR strain, Bacillus subtilis strain JS. After hybridization, forward and reverse subtraction cDNA libraries containing 580 clones were obtained. Blastx search detected 170 single genes. These were classified into 14 functional categories, including metabolic processes and cellular processes implicated in growth promotion. Of the 36 responsive genes confirmed by RT-PCR, expression of 24 genes was up-regulated and 12 were down-regulated by treatment with B. subtilis JS volatile compounds. Photosynthesis pathway related genes encoding chlorophyll a/b binding protein, chloroplast sedoheptulose-1,7-bisphosphatase, and the photosynthate transport related gene were up-regulated, perhaps accounting for the volatile compound-mediated enhanced plant growth. Reactive oxygen species scavenging enzyme encoding genes as well as glutathione S-transferase and methionine-R-sulfoxide reductase were down-regulated.

Keywords

Plant growth-promoting rhizobacteria Suppression subtractive hybridization Volatiles RT-PCR 

Notes

Acknowledgments

This work was supported by a Grant (Code# S211214L030210) from Forest Science & Technology Projects, Forest Service. Republic of Korea.

Conflict of interest

Authors declare no conflict of interest on the contents of the manuscript.

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Copyright information

© The Genetics Society of Korea and Springer-Science and Media 2015

Authors and Affiliations

  • Ji-Seong Kim
    • 1
    • 2
  • Jeongeun Lee
    • 1
    • 2
  • Sang-Gyu Seo
    • 1
  • Chanhui Lee
    • 3
  • Su Young Woo
    • 1
  • Sun-Hyung Kim
    • 1
  1. 1.Department of Environmental HorticultureThe University of SeoulSeoulRepublic of Korea
  2. 2.Graduate School of Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  3. 3.Graduate School of BiotechnologyKyung Hee UniversityYonginRepublic of Korea

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