Genes & Genomics

, Volume 37, Issue 3, pp 263–270 | Cite as

Analysis of DNA methylation in tissues and development stages of pearl oyster Pinctada fucata

Research Article
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Abstract

In this study, we developed a methylation-sensitive amplification polymorphism technique to investigate DNA methylation profiles in different tissues and in the early-development stages of the pearl oyster Pinctada fucata (P. fucata). Methylation levels in adductor muscle, digestive gland, axe foot, heart, and gill ranged from 11.71 to 14.71 %, and significant differences (P < 0.05) between methylation levels in different tissues were observed. The DNA methylation levels of sperm, egg cells, two-cell embryos, morula embryos, trochophore larvae and D-shaped larvae were 13.51, 11.80, 12.14, 12.60, 14.65 and 13.18 %, respectively. Development stages of two-cell embryos, morula embryos, trochophore larvae and D-shaped larvae indicated a higher number of identical DNA methylation status loci in the egg, compared to that in the sperm. It is probable that DNA methylation patterns of the progeny are mainly influenced by the egg, while the sperm may become increasingly important during the process of early embryo development. The observed differences in methylation levels in the tissues and the development stages of P. fucata suggest that DNA methylation may act as an epigenetic regulator during tissue differentiation, individual growth, and development.

Keywords

DNA methylation MSAP Tissue Development stage Pinctada fucata 
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Notes

Acknowledgments

This work was supported by Grants from the National Science and Technology Program of China (2012AA10A410), Marine Fishery Science and Technology Promotion Program of Guangdong Province, China (A201201A05, A201301A03).

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13258_2014_246_MOESM1_ESM.doc (38 kb)
Supplementary material 1 (DOC 38 kb)

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Copyright information

© The Genetics Society of Korea and Springer-Science and Media 2014

Authors and Affiliations

  • Yao-guo Li
    • 1
    • 2
  • Yun-yan Guan
    • 1
  • Qin Li
    • 1
    • 2
  • Mao-xian He
    • 1
  1. 1.CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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